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23 pages, 1910 KiB  
Article
Generation of Mechanical Characteristics in Workpiece Subsurface Layers through Milling
by Michael Storchak, Larysa Hlembotska and Oleksandr Melnyk
Materials 2024, 17(7), 1552; https://doi.org/10.3390/ma17071552 (registering DOI) - 28 Mar 2024
Abstract
The generation of mechanical characteristics in workpiece subsurface layers as a result of the cutting process has a predominant influence on the performance properties of machined parts. The effect of the end milling process on the mechanical characteristics of the machined subsurface layers [...] Read more.
The generation of mechanical characteristics in workpiece subsurface layers as a result of the cutting process has a predominant influence on the performance properties of machined parts. The effect of the end milling process on the mechanical characteristics of the machined subsurface layers was evaluated using nondestructive methods: instrumented nanoindentation and sclerometry (scratching). In this paper, the influence of one of the common processes of materials processing by cutting—the process of end tool milling—on the generation of mechanical characteristics of workpiece machined subsurface layers is studied. The effect of the end milling process on the character of mechanical property formation was evaluated through the coincidence of the cutting process energy characteristics with the mechanical characteristics of the machined subsurface layers. The total cutting power and cutting work in the tertiary cutting zone area were used as energy characteristics of the end milling process. The modes of the end milling process are considered as the main parameters affecting these energy characteristics. The mechanical characteristics of the workpiece machined subsurface layers were the microhardness of the subsurface layers and the total work of indenter penetration, determined by instrumental nanoindentation, and the maximum depth of indenter penetration, determined by sclerometry. Titanium alloy Ti10V2Fe3Al (Ti-1023) was used as the machining material. Based on the evaluation of the coincidence of the cutting process energy characteristics with the specified mechanical characteristics of the machined subsurface layers, the milling mode effect of the studied titanium alloy, in particular the cutter feed and cutting speed, on the generated mechanical characteristics was established. Full article
16 pages, 785 KiB  
Article
A Paternò–Büchi Reaction of Aromatics with Quinones under Visible Light Irradiation
by Wen-Wen Li, Jia-Lin Zhao, Ze-Yu Wang, Pei-Ting Li, Zi-Fa Shi, Xiao-Ping Cao and Qiang Liu
Molecules 2024, 29(7), 1513; https://doi.org/10.3390/molecules29071513 (registering DOI) - 28 Mar 2024
Abstract
Reported herein is a Paternò–Büchi reaction of aromatic double bonds with quinones under visible light irradiation. The reactions of aromatics with quinones exposed to blue LED irradiation yielded oxetanes at −78 °C, which was attributed to both the activation of double bonds in [...] Read more.
Reported herein is a Paternò–Büchi reaction of aromatic double bonds with quinones under visible light irradiation. The reactions of aromatics with quinones exposed to blue LED irradiation yielded oxetanes at −78 °C, which was attributed to both the activation of double bonds in aromatics and the stabilization of oxetanes by thiadiazole, oxadiazole, or selenadiazole groups. The addition of Cu(OTf)2 to the reaction system at room temperature resulted in the formation of diaryl ethers via the copper-catalyzed ring opening of oxetanes in situ. Notably, the substrate scope was extended to general aromatics. Full article
(This article belongs to the Section Organic Chemistry)
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19 pages, 590 KiB  
Article
Biological Control of Pseudomonas syringae in Tomato Using Filtrates and Extracts Produced by Alternaria leptinellae
by Carlos García-Latorre, Sara Rodrigo and Oscar Santamaria
Horticulturae 2024, 10(4), 334; https://doi.org/10.3390/horticulturae10040334 (registering DOI) - 28 Mar 2024
Abstract
Endophytic fungi offer promising alternatives for sustainable plant disease management strategies, often through the production of bioactive secondary metabolites. This study investigated the biocontrol potential of filtrates and extracts, produced under controlled conditions, from Alternaria leptinellae E138 against Pseudomonas syringae in tomato plants [...] Read more.
Endophytic fungi offer promising alternatives for sustainable plant disease management strategies, often through the production of bioactive secondary metabolites. This study investigated the biocontrol potential of filtrates and extracts, produced under controlled conditions, from Alternaria leptinellae E138 against Pseudomonas syringae in tomato plants under greenhouse conditions. To understand the main mechanisms involved in biocontrol, the direct inhibition of bacterial growth and disruption of quorum sensing activity caused by metabolites were studied in vitro, as well as indirect mechanisms, such as their capacity to produce phytohormone-like substances, nutrient mobilization, and antioxidant activity, which can enhance plant growth and fitness. Moreover, a mass spectrometry analysis was used to tentatively identify the secondary metabolites present in the extract with antimicrobial properties, which could explain the biocontrol effects observed. Mycopriming assays, involving the direct treatment of tomato seeds with the fungal A. leptinellae E138 extracts, produced increased germination rates and seedling vigor in tomato seeds. As another treatment, postemergence application of the extracts in greenhouse conditions significantly improved plant health and resulted in a 41% decrease in disease severity. Overall, this study underscores the potential of A. leptinellae E138 extract as a plant growth promoter with biocontrol capabilities, offering promising avenues for sustainable plant disease management. Full article
24 pages, 7995 KiB  
Article
Hybrid Adaptive Impedance and Admittance Control Based on the Sensorless Estimation of Interaction Joint Torque for Exoskeletons: A Case Study of an Upper Limb Rehabilitation Robot
by Auwalu Muhammad Abdullahi, Ado Haruna and Ronnapee Chaichaowarat
J. Sens. Actuator Netw. 2024, 13(2), 24; https://doi.org/10.3390/jsan13020024 (registering DOI) - 28 Mar 2024
Abstract
Physiotherapy is the treatment to recover a patient’s mobility and limb function after an injury, illness, or disability. Rehabilitation robots can be used to replace human physiotherapists. To ensure safety during robot physical therapy, the patient’s limb needs to be controlled to track [...] Read more.
Physiotherapy is the treatment to recover a patient’s mobility and limb function after an injury, illness, or disability. Rehabilitation robots can be used to replace human physiotherapists. To ensure safety during robot physical therapy, the patient’s limb needs to be controlled to track a desired joint trajectory, and the torque due to interaction force/torque needs to be measured and regulated. Therefore, hybrid impedance and admittance with position control (HIPC) is required to track the trajectory and simultaneously regulate the contact torque. The literature describes two structures of HIPC: (1) a switched framework between admittance and impedance control operating in parallel (HIPCSW); and (2) a series connection between admittance and impedance control without switching. In this study, a hybrid adaptive impedance and position-based admittance control (HAIPC) in series is developed, which consists of a proportional derivative-based admittance position controller with gravitational torque compensation and an adaptive impedance controller. An extended state observer is used to estimate the interaction joint torque due to human stiff contact with the exoskeleton without the use of force/torque sensor, which is then used in the adaptive algorithm to update the stiffness and damping gains of the adaptive impedance controller. Simulation results obtained using MATLAB show that the proposed HAIPC significantly reduces the mean absolute values of the actuation torques (control inputs) required for the shoulder and elbow joints in comparison with HIPC and HIPCSW. Full article
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22 pages, 528 KiB  
Article
A Quasilinearization Approach for Identification Control Vectors in Fractional-Order Nonlinear Systems
by Miglena N. Koleva and Lubin G. Vulkov
Fractal Fract. 2024, 8(4), 196; https://doi.org/10.3390/fractalfract8040196 (registering DOI) - 28 Mar 2024
Abstract
This paper is concerned with solving the problem of identifying the control vector problem for a fractional multi-order system of nonlinear ordinary differential equations (ODEs). We describe a quasilinearization approach, based on minimization of a quadratic functional, to compute the values of the [...] Read more.
This paper is concerned with solving the problem of identifying the control vector problem for a fractional multi-order system of nonlinear ordinary differential equations (ODEs). We describe a quasilinearization approach, based on minimization of a quadratic functional, to compute the values of the unknown parameter vector. Numerical algorithm combining the method with appropriate fractional derivative approximation on graded mesh is applied to SIS and SEIR problems to illustrate the efficiency and accuracy. Tikhonov regularization is implemented to improve the convergence. Results from computations, both with noisy-free and noisy data, are provided and discussed. Simulations with real data are also performed. Full article
(This article belongs to the Special Issue Fractional Order Controllers for Non-linear Systems)
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21 pages, 745 KiB  
Systematic Review
Roles and Effect of Digital Technology on Young Children’s STEM Education: A Scoping Review of Empirical Studies
by Xinyun Hu, Yuan Fang and Yutong Liang
Educ. Sci. 2024, 14(4), 357; https://doi.org/10.3390/educsci14040357 (registering DOI) - 28 Mar 2024
Abstract
Digital technology is increasingly used in STEM education for young children aged 0–8 years. An extensive literature search was conducted using seven databases to systematically investigate the effect of digital technology on young children’s STEM education. Twenty-two eligible articles published from 2010 to [...] Read more.
Digital technology is increasingly used in STEM education for young children aged 0–8 years. An extensive literature search was conducted using seven databases to systematically investigate the effect of digital technology on young children’s STEM education. Twenty-two eligible articles published from 2010 to 2021 were identified. Results showed that robotics, programming, and multimedia were used to support young children’s STEM education. Digital technology plays different roles in the process of STEM education. Outcomes also showed that digital technology positively affected young children’s STEM education in terms of STEM knowledge or skill acquisition and learning engagement. This was regardless of gender but relevant to age and the learning condition. Participating children and teachers reported high acceptance and satisfaction with the included programs. However, many difficulties, challenges and criticisms were revealed by the extracted data, including how digital technology is used in young children’s STEM education, the nature of young children, the requirements placed upon educators, and different types of adult–child interactions. We also look at the limitations of the study design within included studies and provide recommendations accordingly. Full article
18 pages, 4304 KiB  
Article
Yeast Diversity in Honey and Pollen Samples from Stingless Bees in the State of Bahia, Brazil: Use of the MALDI-TOF MS/Genbank Proteomic Technique
by Raquel Nunes Almeida da Silva, Karina Teixeira Magalh?es-Guedes, Rogério Marcos de Oliveira Alves, Angélica Cristina Souza, Rosane Freitas Schwan and Marcelo Andrés Umsza-Guez
Microorganisms 2024, 12(4), 678; https://doi.org/10.3390/microorganisms12040678 (registering DOI) - 28 Mar 2024
Abstract
(1) Background: The identification of microorganisms includes traditional biochemical methods, molecular biology methods evaluating the conserved regions of rRNA, and the molecular biology of proteins (proteomics), such as MALDI-TOF MS mass spectrometry. This work aimed to identify the biodiversity of yeasts associated with [...] Read more.
(1) Background: The identification of microorganisms includes traditional biochemical methods, molecular biology methods evaluating the conserved regions of rRNA, and the molecular biology of proteins (proteomics), such as MALDI-TOF MS mass spectrometry. This work aimed to identify the biodiversity of yeasts associated with stingless bee species’ honey and pollen, Melipona scutellaris, Nannotrigona testaceicornes, and Tetragonisca angustula, from the region of São Gonçalo dos Campos-Bahia (BA) state, Brazil. (2) Methods: Cellular proteins were extracted from 2837 microbial isolates (pollen and honey) and identified via MALDI-TOF MS. The identified yeast species were also compared to the mass spectra of taxonomically well-characterized reference strains, available from the National Center of Biotechnology Information (NCBI) database. (3) Results: Nine yeast species were identified: Candida maltosa, Candida norvegica, Kazachstania telluris, Schizosaccharomyces pombe, Scheffersomyces insectosus, Meyerozyma guilliermondii, Brettanomyces bruxellensis, Kazachstania exigua, and Starmerella lactis-condensi. Nannotrigona testaceicornes pollen had the highest number of yeast colonies. The yeasts Brettanomyces bruxellensis and Kazachstania telluris showed high populations in the samples of Nannotrigona testaceicornes and Melipona scutellaris, respectively. This work shows that there is some sharing of the same species of yeast between honey and pollen from the same beehive. (4) Conclusions: A total of 71.84% of the identified species present a high level of confidence at the species level. Eight yeast species (Candida maltosa, Candida norvegica, Kazachstania telluris, Schizosaccharomyces pombe, Scheffersomyces insectosus, Meyerozyma guilliermondii, Kazachstania exigua, and Starmerella lactis-condensi) were found for the first time in the samples that the authors inspected. This contributes to the construction of new knowledge about the diversity of yeasts associated with stingless bee products, as well as to the possibility of the biotechnological application of some yeast species. Full article
(This article belongs to the Section Microbial Biotechnology)
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16 pages, 418 KiB  
Review
Individualized Multimodal Immunotherapy (IMI): Scientific Rationale and Clinical Experience from a Single Institution
by Volker Schirrmacher, Stefaan Van Gool and Wilfried Stuecker
Biomedicines 2024, 12(4), 754; https://doi.org/10.3390/biomedicines12040754 (registering DOI) - 28 Mar 2024
Abstract
Oncolytic viruses and combinatorial immunotherapy for cancer (this Special Issue) are both part of cancer treatment at IOZK. This review focusses on an individual multimodal cancer immunotherapy concept developed by IOZK, Cologne, Germany. The scientific rationale for employing three main components is explained: [...] Read more.
Oncolytic viruses and combinatorial immunotherapy for cancer (this Special Issue) are both part of cancer treatment at IOZK. This review focusses on an individual multimodal cancer immunotherapy concept developed by IOZK, Cologne, Germany. The scientific rationale for employing three main components is explained: (i) oncolytic Newcastle disease virus, (ii) modulated electrohyperthermia and (iii) individual tumor antigen and oncolytic virus modified dendritic cell vaccine (IO-VACR). The strategy involves repeated cancer-immunity cycles evoked in cancer patients by systemic oncolytic virus exposure plus hyperthermia pretreatment to induce immunogenic cell death followed by intradermal IO-VACR vaccination. As an example of the experience at IOZK, we present the latest results from combining the immunotherapy with standard treatment of patients suffering from glioblastoma multiforme. The promising clinical results in terms of overall survival benefit of additional individualized multimodal immunotherapy are presented. The cancer-immunity cycle, as introduced 10 years ago, describes key important steps occurring locally at the sites of both tumor and draining lymph nodes. This view is extended here towards systemic events occuring in blood where immunogenic cell death-induced tumor antigens are transported into the bone marrow. For 20 years it has been known that bone marrow is an antigen-responsive organ in which dendritic cells present tumor antigens to T cells leading to immunological synapse formation, tumor antigen-specific T cell activation and memory T cell formation. Bone marrow is known to be the most prominent source of de novo cellular generation in the body and to play an important role for the storage and maintenance of immunological memory. Its systemic activation is recommended to augment cancer-immunity cycles. Full article
(This article belongs to the Special Issue Oncolytic Viruses and Combinatorial Immunotherapy for Cancer)
18 pages, 3756 KiB  
Article
A Novel Method for Regional Prospecting Based on Modern 3D Graphics
by Zhaolong Xue, Song Wu, Miao Li and Kaiwang Cheng
Minerals 2024, 14(4), 354; https://doi.org/10.3390/min14040354 (registering DOI) - 28 Mar 2024
Abstract
During comprehensive regional prospecting evaluation and delineation of a prospecting target area, various types of data, including geological, geophysical, geochemical, and remote sensing, are usually integrated and visualized in a unified spatial environment, making it convenient for researchers to identify mineralization. To maximize [...] Read more.
During comprehensive regional prospecting evaluation and delineation of a prospecting target area, various types of data, including geological, geophysical, geochemical, and remote sensing, are usually integrated and visualized in a unified spatial environment, making it convenient for researchers to identify mineralization. To maximize the precision of spatial boundaries, the maps traditionally used in prospecting are predominantly in vector formats. However, with the rapid development of modern real-time 3D graphics and computer cartography technology, raster maps can now provide richer detail representation compared to traditional vector maps while still meeting the precision requirements. In this paper, we present a new GPU-based 3D visualization method for spatial data, specifically, two types of bitmap-based maps called dynamic geochemical maps (DGMs) and interactive geological maps (IGMs). A novel software system implementing this method was developed and has been applied in the exploration of the Zhunuo ore district, Tibet, showing large advantages over traditional vector maps. Full article
(This article belongs to the Special Issue Geochemical Exploration for Critical Mineral Resources)
18 pages, 569 KiB  
Review
Towards Lipid from Microalgae: Products, Biosynthesis, and Genetic Engineering
by Yi Xin, Shan Wu, Congcong Miao, Tao Xu and Yandu Lu
Life 2024, 14(4), 447; https://doi.org/10.3390/life14040447 (registering DOI) - 28 Mar 2024
Abstract
Microalgae can convert carbon dioxide into organic matter through photosynthesis. Thus, they are considered as an environment-friendly and efficient cell chassis for biologically active metabolites. Microalgal lipids are a class of organic compounds that can be used as raw materials for food, feed, [...] Read more.
Microalgae can convert carbon dioxide into organic matter through photosynthesis. Thus, they are considered as an environment-friendly and efficient cell chassis for biologically active metabolites. Microalgal lipids are a class of organic compounds that can be used as raw materials for food, feed, cosmetics, healthcare products, bioenergy, etc., with tremendous potential for commercialization. In this review, we summarized the commercial lipid products from eukaryotic microalgae, and updated the mechanisms of lipid synthesis in microalgae. Moreover, we reviewed the enhancement of lipids, triglycerides, polyunsaturated fatty acids, pigments, and terpenes in microalgae via environmental induction and/or metabolic engineering in the past five years. Collectively, we provided a comprehensive overview of the products, biosynthesis, induced strategies and genetic engineering in microalgal lipids. Meanwhile, the outlook has been presented for the development of microalgal lipids industries, emphasizing the significance of the accurate analysis of lipid bioactivity, as well as the high-throughput screening of microalgae with specific lipids. Full article
(This article belongs to the Special Issue Lipid Metabolism, Regulation and Biosynthesis of Microalgae)
16 pages, 975 KiB  
Article
Corn Cropping Systems in Agricultural Soils from the Bajio Region of Guanajuato: Soil Quality Indexes (SQIs)
by Alejandra Sánchez-Guzmán, Héctor Iván Bedolla-Rivera, Eloy Conde-Barajas, María de la Luz Xochilt Negrete-Rodríguez, Marcos Alfonso Lastiri-Hernández, Francisco Paúl Gámez-Vázquez and Dioselina ?lvarez-Bernal
Appl. Sci. 2024, 14(7), 2858; https://doi.org/10.3390/app14072858 (registering DOI) - 28 Mar 2024
Abstract
Agriculture is a sector of great importance for Mexico’s economy, generating employment and contributing significantly to the country’s gross domestic product. The Bajio stands out as one of the most productive agricultural regions in Mexico. However, intensive agricultural practices in this area have [...] Read more.
Agriculture is a sector of great importance for Mexico’s economy, generating employment and contributing significantly to the country’s gross domestic product. The Bajio stands out as one of the most productive agricultural regions in Mexico. However, intensive agricultural practices in this area have caused a progressive deterioration and loss of soil fertility. This study focused on evaluating the quality of soils used for agriculture in the Bajio region of the State of Guanajuato, Mexico. This evaluation, utilised soil quality indexes (SQIs) based on a total of 27 physicochemical, biological and enzymatic indicators. These indicators were selected by means of a principal component analysis (PCA), which allowed for the identification of a minimum set of data. The SQIs developed in this study categorised soils into different quality levels, ranging from low to high, mainly based on the values observed in the biological indicators (SMR and qCO2), which comprised the established SQIs. The inclusion of these biological indicators provides the developed SQIs with greater sensitivity to detect minor disturbances in agricultural soils due to human activity, compared with SQIs consisting only of physicochemical indicators. The developed SQIs can be used to ensure high-quality food production in soils used for corn cultivation under similar conditions, both nationally and internationally. Full article
(This article belongs to the Section Environmental Sciences)
18 pages, 829 KiB  
Article
Power Flow Regulation Effect and Parameter Design Method of Phase-Shifting Transformer
by Weigang Jin, Hangya Liu, Weizhe Zhang and Jiaxin Yuan
Energies 2024, 17(7), 1622; https://doi.org/10.3390/en17071622 (registering DOI) - 28 Mar 2024
Abstract
In the context of carbon peaking and carbon neutrality goals, the integration of a large number of renewable energy sources may trigger significant tidal changes, leading to transmission congestion, wind and light abandonment, power oscillation, voltage and frequency fluctuations, etc. A phase-shifting transformer [...] Read more.
In the context of carbon peaking and carbon neutrality goals, the integration of a large number of renewable energy sources may trigger significant tidal changes, leading to transmission congestion, wind and light abandonment, power oscillation, voltage and frequency fluctuations, etc. A phase-shifting transformer is a cost-effective and reliable power flow control equipment, but its key parameters lack systematic design methods. Based on the equivalent model of phase-shifting transformers, detailed design principles and methods have been proposed, and the configuration method of winding turns has been improved to facilitate calculation and control strategy formulation. The regulation effect of the phase-shifting transformer designed through simulation of power flow regulation in a typical 500 kV network is simulated. By adjusting the phase-shifting transformer, the power flow of heavy load lines can be transferred to light load lines, optimizing the power flow distribution and improving the transmission capacity of the cross-section. Full article
45 pages, 1119 KiB  
Review
CC Chemokine Family Members’ Modulation as a Novel Approach for Treating Central Nervous System and Peripheral Nervous System Injury—A Review of Clinical and Experimental Findings
by Agata Ciechanowska and Joanna Mika
Int. J. Mol. Sci. 2024, 25(7), 3788; https://doi.org/10.3390/ijms25073788 (registering DOI) - 28 Mar 2024
Abstract
Despite significant progress in modern medicine and pharmacology, damage to the nervous system with various etiologies still poses a challenge to doctors and scientists. Injuries lead to neuroimmunological changes in the central nervous system (CNS), which may result in both secondary damage and [...] Read more.
Despite significant progress in modern medicine and pharmacology, damage to the nervous system with various etiologies still poses a challenge to doctors and scientists. Injuries lead to neuroimmunological changes in the central nervous system (CNS), which may result in both secondary damage and the development of tactile and thermal hypersensitivity. In our review, based on the analysis of many experimental and clinical studies, we indicate that the mechanisms occurring both at the level of the brain after direct damage and at the level of the spinal cord after peripheral nerve damage have a common immunological basis. This suggests that there are opportunities for similar pharmacological therapeutic interventions in the damage of various etiologies. Experimental data indicate that after CNS/PNS damage, the levels of 16 among the 28 CC-family chemokines, i.e., CCL1, CCL2, CCL3, CCL4, CCL5, CCL6, CCL7, CCL8, CCL9, CCL11, CCL12, CCL17, CCL19, CCL20, CCL21, and CCL22, increase in the brain and/or spinal cord and have strong proinflammatory and/or pronociceptive effects. According to the available literature data, further investigation is still needed for understanding the role of the remaining chemokines, especially six of them which were found in humans but not in mice/rats, i.e., CCL13, CCL14, CCL15, CCL16, CCL18, and CCL23. Over the past several years, the results of studies in which available pharmacological tools were used indicated that blocking individual receptors, e.g., CCR1 (J113863 and BX513), CCR2 (RS504393, CCX872, INCB3344, and AZ889), CCR3 (SB328437), CCR4 (C021 and AZD-2098), and CCR5 (maraviroc, AZD-5672, and TAK-220), has beneficial effects after damage to both the CNS and PNS. Recently, experimental data have proved that blockades exerted by double antagonists CCR1/3 (UCB 35625) and CCR2/5 (cenicriviroc) have very good anti-inflammatory and antinociceptive effects. In addition, both single (J113863, RS504393, SB328437, C021, and maraviroc) and dual (cenicriviroc) chemokine receptor antagonists enhanced the analgesic effect of opioid drugs. This review will display the evidence that a multidirectional strategy based on the modulation of neuronal–glial–immune interactions can significantly improve the health of patients after CNS and PNS damage by changing the activity of chemokines belonging to the CC family. Moreover, in the case of pain, the combined administration of such antagonists with opioid drugs could reduce therapeutic doses and minimize the risk of complications. Full article
(This article belongs to the Special Issue The Multiple Mechanisms Underlying Neuropathic Pain (III))
19 pages, 3420 KiB  
Article
Blood-Based DNA Methylation Analysis by Multiplexed OBBPA-ddPCR to Verify Indications for Prostate Biopsies in Suspected Prostate Cancer Patients
by Markus Friedemann, Carsten Jandeck, Lars Tautz, Katharina Gutewort, Lisa von Rein, Olga Sukocheva, Susanne Fuessel and Mario Menschikowski
Cancers 2024, 16(7), 1324; https://doi.org/10.3390/cancers16071324 (registering DOI) - 28 Mar 2024
Abstract
Current prostate carcinoma (PCa) biomarkers, including total prostate-specific antigen (tPSA), have unsatisfactory diagnostic sensitivity and specificity resulting in overdiagnosis and overtreatment. Previously, we described an optimised bias-based preamplification–digital droplet PCR (OBBPA-ddPCR) technique, which detects tumour DNA in blood-derived cell-free DNA (cfDNA) of cancer [...] Read more.
Current prostate carcinoma (PCa) biomarkers, including total prostate-specific antigen (tPSA), have unsatisfactory diagnostic sensitivity and specificity resulting in overdiagnosis and overtreatment. Previously, we described an optimised bias-based preamplification–digital droplet PCR (OBBPA-ddPCR) technique, which detects tumour DNA in blood-derived cell-free DNA (cfDNA) of cancer patients. The current study investigated the performance of newly developed OBBPA-ddPCR-based biomarkers. Blood plasma samples from healthy individuals (n = 90, controls) and PCa (n = 39) and benign prostatic hyperplasia patients (BPH, n = 40) were analysed. PCa and BPH patients had tPSA values within a diagnostic grey area of 2–15 ng/mL, for whom further diagnostic validation is most crucial. Methylation levels of biomarkers RASSF1A, MIR129-2, NRIP3, and SOX8 were found significantly increased in PCa patients compared to controls. By combining classical PCa risk factors (percentage of free PSA compared to tPSA (QfPSA) and patient’s age) with cfDNA-based biomarkers, we developed PCa risk scores with improved sensitivity and specificity compared to established tPSA and QfPSA single-marker analyses. The diagnostic specificity was increased to 70% with 100% sensitivity for clinically significant PCa patients. Thus, prostate biopsies could be avoided for 28 out of 40 BPH patients. In conclusion, the newly developed risk scores may help to confirm the clinical decision and prevent unnecessary prostate biopsy. Full article
(This article belongs to the Section Cancer Pathophysiology)
23 pages, 4184 KiB  
Article
Spatial Dynamics and Social Order in Traditional Towns of Saudi Arabia’s Nadji Region: The Role of Neighborhood Clustering in Urban Morphology and Decision-Making Processes
by Mohammed Mashary Alnaim and Emad Noaime
Sustainability 2024, 16(7), 2830; https://doi.org/10.3390/su16072830 (registering DOI) - 28 Mar 2024
Abstract
This study explores how the layout of neighborhoods in traditional settlements of Saudi Arabia’s Najdi region influence social interactions and urban planning decisions. The study uses a multidisciplinary approach that includes urban morphology, architectural phenomenology, and sociological study methods to investigate the relationships [...] Read more.
This study explores how the layout of neighborhoods in traditional settlements of Saudi Arabia’s Najdi region influence social interactions and urban planning decisions. The study uses a multidisciplinary approach that includes urban morphology, architectural phenomenology, and sociological study methods to investigate the relationships between spatial organization and decision-making processes on both the macro and micro levels of decision-making. The purpose is to look at how collective action decision-making processes affect the urban fabric and how social norms influence spatial organization at different levels. The study applies case study and spatial analysis approaches to investigate how the traditional settlements’ spatial structure promotes peace among the inhabitants while also sustaining cultural traditions. The qualitative approach investigates how spatial arrangements influence behaviors, developing a better understanding of how residents interact with their surroundings. According to the study’s findings, these spatial layouts sustain customs and assist communities in adapting to environmental changes by retaining cultural activities. The study identifies the significance of balancing development with the retention of important traditional values in the implementation of long-term urban conservation plans. Traditional Najdi towns can serve as urban design examples, emphasizing the need to acknowledge the distinct value of vernacular architecture in modern urban development while also fostering social cohesion. Full article
(This article belongs to the Special Issue Architecture, Urban Space and Heritage in the Digital Age)
20 pages, 354 KiB  
Article
UK Dog Owners’ Pre-Acquisition Information- and Advice-Seeking: A Mixed Methods Study
by Rebecca Mead, Katrina E. Holland, Rachel A. Casey, Melissa M. Upjohn and Robert M. Christley
Animals 2024, 14(7), 1033; https://doi.org/10.3390/ani14071033 (registering DOI) - 28 Mar 2024
Abstract
Dogs are the most common pet animal species in the UK. Little is known about information and advice gathering within the process of dog acquisition, nor what pre-acquisition research encompasses. This study aimed to better understand the preparatory research undertaken by prospective dog [...] Read more.
Dogs are the most common pet animal species in the UK. Little is known about information and advice gathering within the process of dog acquisition, nor what pre-acquisition research encompasses. This study aimed to better understand the preparatory research undertaken by prospective dog owners in the UK. A 2019 online survey collected quantitative and qualitative data about dog acquisition. Analyses within this study focused on a subset of these current (n = 4381) and potential (n = 2350) owners who had undertaken research, or who reported planning to do so, before acquiring a dog. Additional qualitative data were collected through semi-structured interviews with current (n = 24) and potential (n = 8) dog owners. Among those current owners who had sought information or advice, websites were the most common source of information (76%), followed by family and friends (70%), and online forums (51%). Similar patterns were seen among potential owners. Qualitative data revealed that information was commonly sought on the following: general information about dogs; information about breeds or types of dogs; owner requirements and dog suitability; aspects of dog ownership; and how to source a dog. The majority of prospective owners stated that they had found all the information they wanted (96% of current owners and 90% of potential owners) but some respondents found conflicting advice from different sources and did not know which sources to trust. Our study shows that, for some prospective owners, research into various aspects of dog acquisition and ownership is important. Knowing where to look for correct and unbiased advice, particularly online, is particularly important. Understanding the pre-acquisition research that owners undertake, including the sources they use, information they are interested in finding, and the challenges they face, is of interest to animal welfare and veterinary organisations and those involved in rehoming and selling dogs. This information may help inform interventions aimed at improving the knowledge and decisions made by potential dog owners. Full article
19 pages, 2106 KiB  
Article
Assessment of Silver Nanoparticles Derived from Brown Algae Sargassum vulgare: Insight into Antioxidants, Anticancer, Antibacterial and Hepatoprotective Effect
by Ragaa A. Hamouda and Ebtehail S. Aljohani
Mar. Drugs 2024, 22(4), 154; https://doi.org/10.3390/md22040154 (registering DOI) - 28 Mar 2024
Abstract
Algae are used as safe materials to fabricate novel nanoparticles to treat some diseases. Marine brown alga Sargassum vulgare are used to fabricate silver nanoparticles (Sv/Ag-NPs). The characterization of Sv/Ag-NPs was determined by TEM, EDX, Zeta potential, XRD, and UV spectroscopy. The Sv/Ag-NPs [...] Read more.
Algae are used as safe materials to fabricate novel nanoparticles to treat some diseases. Marine brown alga Sargassum vulgare are used to fabricate silver nanoparticles (Sv/Ag-NPs). The characterization of Sv/Ag-NPs was determined by TEM, EDX, Zeta potential, XRD, and UV spectroscopy. The Sv/Ag-NPs were investigated as antioxidant, anticancer, and antibacterial activities against Gram-positive bacteria Bacillus mojavensis PP400982, Staphylococcus caprae PP401704, Staphylococcus capitis PP402689, and Staphylococcus epidermidis PP403851. The activity of the Sv/Ag-NPs was evaluated as hepatoprotective in vitro in comparison with silymarin. The UV–visible spectrum of Sv/Ag-NPs appeared at 442 nm; the size of Sv/Ag-NPs is in range between 6.90 to 16.97 nm, and spherical in shape. Different concentrations of Sv/Ag-NPs possessed antioxidant, anticancer activities against (HepG-2), colon carcinoma (HCT-116), cervical carcinoma (HeLa), and prostate carcinoma (PC-3) with IC50 50.46, 45.84, 78.42, and 100.39 µg/mL, respectively. The Sv/Ag-NPs induced the cell viability of Hep G2 cells and hepatocytes treated with carbon tetrachloride. The Sv/Ag-NPs exhibited antibacterial activities against Staphylococcus caprae PP401704, Staphylococcus capitis PP402689, and Staphylococcus epidermidis PP403851. This study strongly suggests the silver nanoparticles derived from Sargassum vulgare showed potential hepato-protective effect against carbon tetrachloride-induced liver cells, and could be used as anticancer and antibacterial activities. Full article
(This article belongs to the Special Issue Nanoparticle Synthesis with Marine Substances 2.0)
14 pages, 1984 KiB  
Article
Image Filtering to Improve Maize Tassel Detection Accuracy Using Machine Learning Algorithms
by Eric Rodene, Gayara Demini Fernando, Ved Piyush, Yufeng Ge, James C. Schnable, Souparno Ghosh and Jinliang Yang
Sensors 2024, 24(7), 2172; https://doi.org/10.3390/s24072172 (registering DOI) - 28 Mar 2024
Abstract
Unmanned aerial vehicle (UAV)-based imagery has become widely used to collect time-series agronomic data, which are then incorporated into plant breeding programs to enhance crop improvements. To make efficient analysis possible, in this study, by leveraging an aerial photography dataset for a field [...] Read more.
Unmanned aerial vehicle (UAV)-based imagery has become widely used to collect time-series agronomic data, which are then incorporated into plant breeding programs to enhance crop improvements. To make efficient analysis possible, in this study, by leveraging an aerial photography dataset for a field trial of 233 different inbred lines from the maize diversity panel, we developed machine learning methods for obtaining automated tassel counts at the plot level. We employed both an object-based counting-by-detection (CBD) approach and a density-based counting-by-regression (CBR) approach. Using an image segmentation method that removes most of the pixels not associated with the plant tassels, the results showed a dramatic improvement in the accuracy of object-based (CBD) detection, with the cross-validation prediction accuracy (r2) peaking at 0.7033 on a detector trained with images with a filter threshold of 90. The CBR approach showed the greatest accuracy when using unfiltered images, with a mean absolute error (MAE) of 7.99. However, when using bootstrapping, images filtered at a threshold of 90 showed a slightly better MAE (8.65) than the unfiltered images (8.90). These methods will allow for accurate estimates of flowering-related traits and help to make breeding decisions for crop improvement. Full article
(This article belongs to the Special Issue AI-Driven Sensing for Image Processing and Recognition)
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48 pages, 8619 KiB  
Review
Global Occurrence, Geology and Characteristics of Hydrothermal-Origin Kaolin Deposits
by ?mer I??k Ece and Hatice ?nal Ercan
Minerals 2024, 14(4), 353; https://doi.org/10.3390/min14040353 (registering DOI) - 28 Mar 2024
Abstract
Kaolin-group minerals occur in nature as the result of high-sulfidation acid sulfate, sulfur-poor HCl-, HF- and H2CO3-rich acidic fluid-related hydrothermal alterations and in situ geochemical weathering. These minerals possess different crystallographic and chemical properties that determine their application areas, [...] Read more.
Kaolin-group minerals occur in nature as the result of high-sulfidation acid sulfate, sulfur-poor HCl-, HF- and H2CO3-rich acidic fluid-related hydrothermal alterations and in situ geochemical weathering. These minerals possess different crystallographic and chemical properties that determine their application areas, mainly in the ceramic and paper industries, and as nanocomposite materials. The physicochemical properties of hydrothermal kaolin deposits are the result of the type of parent rock, the effect of the regional tectonism-associated magmatism, and the chemical features of hydrothermal fluids that interact with the deep basement rocks. However, understanding these geothermal systems is one of the most challenging issues due to the rich mineralogical assemblages, complex geochemistry and isotopic data of hydrothermal alteration zones. This study evaluates the formation of hydrothermal-origin kaolin-group minerals by considering their characteristics of hydrothermal alteration, isotopic compositions and differences in characteristic properties of low- and high-sulfidation occurrences; this paper also addresses mineralogical and structural differences between hypogene and supergene kaolin formations, and kaolin–alunite–pyrophyllite association, and it provides examples of worldwide occurrences. The study of the mineralogical assemblages, geochemistry and isotopic data of the hydrothermal alteration zones is one of the most challenging subjects in terms of gaining a detailed understanding of the geothermal systems. Silicification processes are subsequent to late-stage alteration after the completion of kaolinization processes, erasing existing hydrothermal mineralogical and geochemical traces and making interpretation difficult. In the early stages involving magmatic–hydrothermal-origin acidic geothermal fluids, the latter comes from the disproportionation of SO2 (+H2O) and H2S oxidation to H2SO4 in hydrothermal environments. In the later stages, due to spatial and temporal changes over time in the chemistry of geothermal fluids, the system comes to have a more alkali–chloride composition, with neutral pH waters frequently saturated with amorphous silica which characteristically precipitate as siliceous sinter deposits containing large amounts of opal-A. Full article
(This article belongs to the Section Mineral Deposits)
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20 pages, 1256 KiB  
Article
The Dual Role of Neutrophil Extracellular Traps (NETs) in Sepsis and Ischemia-Reperfusion Injury: Comparative Analysis across Murine Models
by Antonia Kiwit, Yuqing Lu, Moritz Lenz, Jasmin Knopf, Christoph Mohr, Yannick Ledermann, Michaela Klinke-Petrowsky, Laia Pagerols Raluy, Konrad Reinshagen, Martin Herrmann, Michael Boettcher and Julia Elrod
Int. J. Mol. Sci. 2024, 25(7), 3787; https://doi.org/10.3390/ijms25073787 (registering DOI) - 28 Mar 2024
Abstract
A better understanding of the function of neutrophil extracellular traps (NETs) may facilitate the development of interventions for sepsis. The study aims to investigate the formation and degradation of NETs in three murine sepsis models and to analyze the production of reactive oxygen [...] Read more.
A better understanding of the function of neutrophil extracellular traps (NETs) may facilitate the development of interventions for sepsis. The study aims to investigate the formation and degradation of NETs in three murine sepsis models and to analyze the production of reactive oxygen species (ROS) during NET formation. Murine sepsis was induced by midgut volvulus (720° for 15 min), cecal ligation and puncture (CLP), or the application of lipopolysaccharide (LPS) (10 mg/kg body weight i.p.). NET formation and degradation was modulated using mice that were genetically deficient for peptidyl arginine deiminase-4 (PAD4-KO) or DNase1 and 1L3 (DNase1/1L3-DKO). After 48 h, mice were killed. Plasma levels of circulating free DNA (cfDNA) and neutrophil elastase (NE) were quantified to assess NET formation and degradation. Plasma deoxyribonuclease1 (DNase1) protein levels, as well as tissue malondialdehyde (MDA) activity and glutathione peroxidase (GPx) activity, were quantified. DNase1 and DNase1L3 in liver, intestine, spleen, and lung tissues were assessed. The applied sepsis models resulted in a simultaneous increase in NET formation and oxidative stress. NET formation and survival differed in the three models. In contrast to LPS and Volvulus, CLP-induced sepsis showed a decreased and increased 48 h survival in PAD4-KO and DNase1/1L3-DKO mice, when compared to WT mice, respectively. PAD4-KO mice showed decreased formation of NETs and ROS, while DNase1/1L3-DKO mice with impaired NET degradation accumulated ROS and chronicled the septic state. The findings indicate a dual role for NET formation and degradation in sepsis and ischemia-reperfusion (I/R) injury: NETs seem to exhibit a protective capacity in certain sepsis paradigms (CLP model), whereas, collectively, they seem to contribute adversely to scenarios where sepsis is combined with ischemia-reperfusion (volvulus). Full article
(This article belongs to the Special Issue Recent Advances in Neutrophil Extracellular Traps (NETs))
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34 pages, 2218 KiB  
Systematic Review
Farmers’ Transition to Climate-Smart Agriculture: A Systematic Review of the Decision-Making Factors Affecting Adoption
by Marilena Gemtou, Konstantina Kakkavou, Evangelos Anastasiou, Spyros Fountas, Soren Marcus Pedersen, Gohar Isakhanyan, Kassa Tarekegn Erekalo and Serafin Pazos-Vidal
Sustainability 2024, 16(7), 2828; https://doi.org/10.3390/su16072828 (registering DOI) - 28 Mar 2024
Abstract
Agriculture is currently facing major challenges related to ensuring the food security of a rising population and climate change with extreme weather patterns. At the same time, agriculture is a cause of environmental degradation, pollution and biodiversity loss. Climate-smart agriculture (CSA) is proposed [...] Read more.
Agriculture is currently facing major challenges related to ensuring the food security of a rising population and climate change with extreme weather patterns. At the same time, agriculture is a cause of environmental degradation, pollution and biodiversity loss. Climate-smart agriculture (CSA) is proposed as an approach that provides a roadmap to sustainable agricultural development. Despite this, farmer adoption rates of CSA practices and technologies in Europe remain low. This paper seeks to systematically review and synthesize the factors that facilitate or hinder farmers’ uptake of CSA in Europe. Out of the 2827 articles identified in the Web of Science and Scopus databases, a total of 137 research articles were included for analysis following the PRISMA methodology. The factors are categorized into seven categories, namely socio-demographics, psychological, farm characteristics, practice/technology-related attributes, biotic/abiotic, systemic and policy factors, with the majority of the studies focusing on the first four categories, while systemic and policy factors are relatively understudied. The results highlight for the first time that the adoption of CSA does not depend solely on farmer characteristics but also on the food systems and structures in which farmers operate, as well as the interactions with other value chain actors. To promote the adoption of CSA practices, extension and advisory services along with access to timely and reliable information, play a vital role in increasing awareness and in the provision of training and the encouragement of farmers’ behavioral shifts towards sustainable practices. From a technological point of view, adapting technologies to be easy to use, compatible with current farming objectives and cost-efficient will render them less risky investments for farmers and will foster adoption rates. Finally, support from the government in terms of financial support, subsidies and reduced bureaucratic procedures is crucial for motivating CSA adoption. Full article
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9 pages, 535 KiB  
Editorial
Consumer Behavior and Sustainable Marketing Development in Online and Offline Settings
by Sikandar Ali Qalati, Belem Barbosa and Pankaj Deshwal
Sustainability 2024, 16(7), 2829; https://doi.org/10.3390/su16072829 (registering DOI) - 28 Mar 2024
Abstract
The changing dynamics of consumer behavior in today’s digitally driven market presents opportunities and challenges for sustainable marketing development [...] Full article
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25 pages, 3578 KiB  
Article
Operating Properties of Deep Hole Boring Tools with Modified Design
by Norbert K?pczak, Grzegorz Bechciński and Rados?aw Rosik
Materials 2024, 17(7), 1551; https://doi.org/10.3390/ma17071551 (registering DOI) - 28 Mar 2024
Abstract
This paper presents the results of research work on the revised design of a deep hole boring tool. The study was divided into three stages: theoretical, experimental and operational. In the theoretical part, a 3D model of the actual boring bar was created, [...] Read more.
This paper presents the results of research work on the revised design of a deep hole boring tool. The study was divided into three stages: theoretical, experimental and operational. In the theoretical part, a 3D model of the actual boring bar was created, which was subjected to strength tests using the Finite Element Method (FEM), and then prototypes of new deep hole boring tools were made with structural modifications to the shank part of the tool. For the polymer concrete core of a shank, there was a 14.59% lower displacement, and for the rubber-doped polymer concrete (SBR—styrene butadiene rubber) core of a shank there was a 4.84% lower displacement in comparison to the original boring bar. In the experimental part of the study, the original boring bar and the prototypes were subjected to experimental modal analysis and static analysis tests to compare dynamic and static properties. In the operational part of the study, boring tests were carried out for various workpiece materials, during which the basic parameters of the surface geometric structure (SGS), such as roughness Ra and Rz, were studied. Despite the promising preliminary results of the theoretical and experimental studies, using the described modifications to the boring bar is not recommended. Full article
(This article belongs to the Section Manufacturing Processes and Systems)

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