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13 pages, 2035 KiB  
Review
How Hydrogen (H2) Can Support Food Security: From Farm to Fork
by Grace Russell, Alexander Nenov and John T. Hancock
Appl. Sci. 2024, 14(7), 2877; https://doi.org/10.3390/app14072877 (registering DOI) - 29 Mar 2024
Abstract
Molecular hydrogen (H2) is a low-molecular-weight, non-polar and electrochemically neutral substance that acts as an effective antioxidant and cytoprotective agent, with research into the effects of H2 incorporation into the food chain, at various stages, rapidly gaining momentum. H2 [...] Read more.
Molecular hydrogen (H2) is a low-molecular-weight, non-polar and electrochemically neutral substance that acts as an effective antioxidant and cytoprotective agent, with research into the effects of H2 incorporation into the food chain, at various stages, rapidly gaining momentum. H2 can be delivered throughout the food growth, production, delivery and storage systems in numerous ways, including as a gas, as hydrogen-rich water (HRW), or with hydrogen-donating food supplements such as calcium (Ca) or magnesium (Mg). In plants, H2 can be exploited as a seed-priming agent, during seed germination and planting, during the latter stages of plant development and reproduction, as a post-harvest treatment and as a food additive. Adding H2 during plant growth and developmental stages is noted to improve the yield and quality of plant produce, through modulating antioxidant pathways and stimulating tolerance to such environmental stress factors as drought stress, enhanced tolerance to herbicides (paraquat), and increased salinity and metal toxicity. The benefits of pre- and post-harvest application of H2 include reductions in natural senescence and microbial spoilage, which contribute to extending the shelf-life of animal products, fruits, grains and vegetables. This review collates empirical findings pertaining to the use of H2 in the agri-food industry and evaluates the potential impact of this emerging technology. Full article
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12 pages, 788 KiB  
Article
Myelodysplastic Neoplasms (MDS) with Ring Sideroblasts or SF3B1 Mutations: The Improved Clinical Utility of World Health Organization and International Consensus Classification 2022 Definitions, a Single-Centre Retrospective Chart Review
by Shamim Mortuza, Benjamin Chin-Yee, Tyler E. James, Ian H. Chin-Yee, Benjamin D. Hedley, Jenny M. Ho, Lalit Saini, Alejandro Lazo-Langner, Laila Schenkel, Pratibha Bhai, Bekim Sadikovic, Jonathan Keow, Nikhil Sangle and Cyrus C. Hsia
Curr. Oncol. 2024, 31(4), 1762-1773; https://doi.org/10.3390/curroncol31040134 (registering DOI) - 29 Mar 2024
Abstract
Myelodysplastic neoplasms (MDS) with ring sideroblasts (RS) are diagnosed via bone marrow aspiration in the presence of either (i) ≥15% RS or (ii) 5–14% RS and an SF3B1 mutation. In the MEDALIST trial and in an interim analysis of the COMMANDS trial, lower-risk [...] Read more.
Myelodysplastic neoplasms (MDS) with ring sideroblasts (RS) are diagnosed via bone marrow aspiration in the presence of either (i) ≥15% RS or (ii) 5–14% RS and an SF3B1 mutation. In the MEDALIST trial and in an interim analysis of the COMMANDS trial, lower-risk MDS-RS patients had decreased transfusion dependency with luspatercept treatment. A total of 6817 patients with suspected hematologic malignancies underwent molecular testing using a next-generation-sequencing-based genetic assay and 395 MDS patients, seen at our centre from 1 January 2018 to 31 May 2023, were reviewed. Of these, we identified 39 evaluable patients as having lower-risk MDS with SF3B1 mutations: there were 20 (51.3%) males and 19 (48.7%) females, with a median age of 77 years (range of 57 to 92). Nineteen (48.7%) patients had an isolated SF3B1 mutation with a mean variant allele frequency of 35.2% +/− 8.1%, ranging from 7.4% to 46.0%. There were 29 (74.4%) patients with ≥15% RS, 6 (15.4%) with 5 to 14% RS, one (2.6%) with 1% RS, and 3 (7.7%) with no RS. Our study suggests that a quarter of patients would be missed based on the morphologic criterion of only using RS greater than 15% and supports the revised 2022 definitions of the World Health Organization (WHO) and International Consensus Classification (ICC), which shift toward molecularly defined subtypes of MDS and appropriate testing. Full article
(This article belongs to the Special Issue The Molecular Pathology of Myelodysplastic Syndromes)
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13 pages, 220 KiB  
Article
Addressing a Critical Voice in Clinical Practice: Experiences of Nursing Students, Teachers, and Supervisors—A Qualitative Study
by Ingrid Rachel Strand, Unni Knutstad, Anton Havnes and Mette Sagbakken
Nurs. Rep. 2024, 14(2), 788-800; https://doi.org/10.3390/nursrep14020061 (registering DOI) - 29 Mar 2024
Abstract
Aim: Our goal was to explore how power asymmetry manifests within the relationships between students, teachers, and supervisors, and how it influences students’ ability for critical reflection. Design: This study has an explorative qualitative design. Methods: Thirty in-depth interviews with nursing students (15), [...] Read more.
Aim: Our goal was to explore how power asymmetry manifests within the relationships between students, teachers, and supervisors, and how it influences students’ ability for critical reflection. Design: This study has an explorative qualitative design. Methods: Thirty in-depth interviews with nursing students (15), teachers (9), and supervisors (6) were conducted in addition to 16 observations of mid-term assessments during clinical practice. The analysis was conducted using Braun and Clarke’s thematic analysis. Results: The students described being a student as a balancing act between humility, conforming to the supervisor’s expectations, and speaking their minds. The view expressed by the teachers and supervisors is that training for the nursing profession is closely linked to the students’ ability to act independently. Due to the supervisors’ hierarchical position, however, students are hesitant to voice any criticism regarding insufficient supervision or unsatisfactory performance of clinical tasks while at the same time being evaluated on their ability to critically reflect on their own and others’ clinical performance. This study was prospectively registered with the Norwegian Centre for Research Data on the 15th of August 2017 with the registration number 54821. Full article
11 pages, 1174 KiB  
Article
Metal and Pesticide Assessments of Imported and Locally Cultivated Rice (Oryza sativa) in Senegal
by Anna Ndiaye, Alassane Traore, Papa Sam Gueye, Zachary Senwo, Momar Ndiaye and Abdoulaye Diop
Appl. Sci. 2024, 14(7), 2876; https://doi.org/10.3390/app14072876 (registering DOI) - 29 Mar 2024
Abstract
Identifying heavy metal and pesticide contaminants is an essential step in assessing the health indicators of rice cultivation and consumption in Africa. Information on the contaminant levels of the imported and cultivated rice consumed in Senegal seems lacking. In this study, we assessed [...] Read more.
Identifying heavy metal and pesticide contaminants is an essential step in assessing the health indicators of rice cultivation and consumption in Africa. Information on the contaminant levels of the imported and cultivated rice consumed in Senegal seems lacking. In this study, we assessed heavy metals, pesticides, ash, and protein in rice using rice samples from India, Thailand, South America, Vietnam, and China. Arsenic, Pb, Cd, Ni, Cu, Mo, Co, Cr, and Al are usually found in the soils used for rice cultivation in northern Senegal. While the heavy metal levels measured in soils were above the threshold limit, only Pb, Cd, and Al were found in cultivated rice. In all the analyzed rice samples from each country, there were certain amounts of Pb, As Al, and Cd. The concentration ranges in the six countries were as follows: 0.635–1.165 mg kg−1 for Pb, 0.047–0.438 mg kg−1 for As, 2.22–95.54 mg kg−1 for Al, and 0.002–0.082 mg kg−1 for Cd. The protein content in cultivated rice in Senegal was 7.31 mg kg−1, while the average from the imported rice ranged between 6.42% and 7.32%. The humidity levels in imported rice ranged between 11.12% and 12.95%. The fat content for the rice from six countries ranged between 0.22% and 0.67%, and the ash content ranged between 0.23% and 0.48%. These results allowed for the determination of the carbohydrate concentration, which varied between 79.18% and 80.82%. Indeed, freshly harvested rice grains typically contain around 80% carbohydrates. We noticed the presence of pesticides in all rice samples. The levels of three pesticides (total Pyrethrin, Bensulfuron-methyl, Propanyl, and 2,4D) were found to be beyond their maximum residue limits (MRLs) from the Codex Alimentarius, whereas deltamethrin was found to be below its MRL. This study indicates the presence of heavy metals carcinogenic to humans (Al, As, Cd, and Pb). Additionally, this study reveals the presence of deltamethrin, which is classified as probably carcinogenic to humans (Group 1), and 2,4-dichlorophenoxyacetic acid, which is classified as possibly carcinogenic to humans (Group 2B). Full article
(This article belongs to the Section Agricultural Science and Technology)
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15 pages, 2867 KiB  
Article
Determination of the Mass Fractions of the Heavy Metals in the Recycled Cellulose Pulp
by Mia Klemen?i?, Ivana Bolan?a Mirkovi?, Nenad Bolf and Marinko Marki?
Polymers 2024, 16(7), 934; https://doi.org/10.3390/polym16070934 (registering DOI) - 29 Mar 2024
Abstract
In the process of paper recycling, certain amounts of metals can be found in the cellulose suspension, the source of which is mainly printing inks. The paper industry often uses different technologies to reduce heavy metal emissions. The recycling of laminated packaging contributes [...] Read more.
In the process of paper recycling, certain amounts of metals can be found in the cellulose suspension, the source of which is mainly printing inks. The paper industry often uses different technologies to reduce heavy metal emissions. The recycling of laminated packaging contributes to the formation of sticky particles, which affects the concentration of heavy metals. This study aimed to determine the mass fraction of metals in the different phases of the deinking process to optimize the cellulose pulp’s quality and design healthy correct packaging products. In this research, the deinking flotation of laminated and non-laminated samples was carried out by the Ingede 11 method. As a result of the study, the mass fractions of metals in cellulose pulp were divided into four groups according to the mass fraction’s increasing value and the metals’ increasing electronegativity. The quantities of metals were analyzed using Inductively Coupled Mass Spectrometry (ICP-MS). The separation of metals from cellulose pulp is influenced by the presence of adhesives and the electronegativity of the metal. The results of the study show that the recycling process removes certain heavy metals very well, which indicates the good recycling potential of pharmaceutical cardboard samples. Full article
(This article belongs to the Special Issue Advanced Preparation and Application of Cellulose)
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13 pages, 1638 KiB  
Article
Gold Nanoprobes for Detection of a Crucial EGFR Deletion for Early Diagnosis of Non-Small-Cell Lung Cancer
by Maria Enea, Anupong Nuekaew, Ricardo Franco and Eulália Pereira
Biosensors 2024, 14(4), 162; https://doi.org/10.3390/bios14040162 (registering DOI) - 29 Mar 2024
Abstract
Gold nanoparticles (AuNPs) exhibit improved optical and spectral properties compared to bulk materials, making them suitable for the detection of DNA, RNA, antigens, and antibodies. Here, we describe a simple, selective, and rapid non-cross linking detection assay, using approx. 35 nm spherical Au [...] Read more.
Gold nanoparticles (AuNPs) exhibit improved optical and spectral properties compared to bulk materials, making them suitable for the detection of DNA, RNA, antigens, and antibodies. Here, we describe a simple, selective, and rapid non-cross linking detection assay, using approx. 35 nm spherical Au nanoprobes, for a common mutation occurring in exon 19 of the epidermal growth factor receptor (EGFR), associated with non-small-cell lung cancer cells. AuNPs were synthesized based on the seed-mediated growth method and functionalized with a specific 16 bp thiolated oligonucleotide using a pH-assisted method. Both AuNPs and Au nanoprobes proved to be highly stable and monodisperse through ultraviolet-visible spectrophotometry, dynamic light scattering (DLS), and electrophoretic light scattering (ELS). Our results indicate a detection limit of 1.5 µg mL−1 using a 0.15 nmol dm−3 Au nanoprobe concentration. In conclusion, this work presents an effective possibility for a straightforward, fast, and inexpensive alternative for the detection of DNA sequences related to lung cancer, leading to a potential platform for early diagnosis of lung cancer patients. Full article
(This article belongs to the Special Issue Recent Advances in Nanomaterial-Based Biosensing and Diagnosis)
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14 pages, 1854 KiB  
Article
Brewing Mainly from Stale Bread: A Pale Ale Case Study
by Pedro Coelho, Catarina Prista and Isabel Sousa
Beverages 2024, 10(2), 23; https://doi.org/10.3390/beverages10020023 (registering DOI) - 29 Mar 2024
Abstract
Contemporary environmental concerns have led to the prioritization of sustainable production and material circularity, no matter what the industrial field of activity. Bread waste is a major element of overall food waste since, worldwide, bread remains a widespread staple food. A considerable proportion [...] Read more.
Contemporary environmental concerns have led to the prioritization of sustainable production and material circularity, no matter what the industrial field of activity. Bread waste is a major element of overall food waste since, worldwide, bread remains a widespread staple food. A considerable proportion of bread consumption is of fresh, baked bread, consumed daily, generating substantial amounts of stale bread. Therefore, efforts to reintroduce this waste into the food value chain can make a significant contribution to reaching zero food waste, which is a major target in European countries. Possible ways to produce new raw materials through starch enzymatic hydrolysis include brewing, which is an activity in which incorporating stale bread is of great interest. Mashing parameters in brewing processing are the main focus of this study, primarily the time and temperature required to acquire optimal enzymatic activity for starch-efficient hydrolysis. Extending the mashing time to 290 min, within a temperature range of 45–75 °C, allowed us to replace 50% of the required malt with stale bread, thus obtaining a successful pale ale beer. The incorporation of stale bread in a 50:50 ratio did not affect the overall character of the beer, although the alcohol levels stood around 2% below a standard beer’s average level. Depending on the brewer’s final goal, this lighter kind of beer may be well-aligned with new consumer trends supporting more sustainable and lower-alcohol beverages. Full article
(This article belongs to the Section Malting, Brewing and Beer)
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12 pages, 3303 KiB  
Article
Fabrication of Large-Area Silicon Spherical Microlens Arrays by Thermal Reflow and ICP Etching
by Yu Wu, Xianshan Dong, Xuefang Wang, Junfeng Xiao, Quanquan Sun, Lifeng Shen, Jie Lan, Zhenfeng Shen, Jianfeng Xu and Yuqingyun Du
Micromachines 2024, 15(4), 460; https://doi.org/10.3390/mi15040460 (registering DOI) - 29 Mar 2024
Abstract
In this paper, we proposed an efficient and high-precision process for fabricating large-area microlens arrays using thermal reflow combined with ICP etching. When the temperature rises above the glass transition temperature, the polymer cylinder will reflow into a smooth hemisphere due to the [...] Read more.
In this paper, we proposed an efficient and high-precision process for fabricating large-area microlens arrays using thermal reflow combined with ICP etching. When the temperature rises above the glass transition temperature, the polymer cylinder will reflow into a smooth hemisphere due to the surface tension effect. The dimensional differences generated after reflow can be corrected using etching selectivity in the following ICP etching process, which transfers the microstructure on the photoresist to the substrate. The volume variation before and after reflow, as well as the effect of etching selectivity using process parameters, such as RF power and gas flow, were explored. Due to the surface tension effect and the simultaneous molding of all microlens units, machining a 3.84 × 3.84 mm2 silicon microlens array required only 3 min of reflow and 15 min of ICP etching with an extremely low average surface roughness Sa of 1.2 nm. Full article
(This article belongs to the Special Issue Advanced Manufacturing Technology and Systems, 3rd Edition)
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10 pages, 226 KiB  
Article
A Delphi Study on Identifying Competencies in Virtual Healthcare for Healthcare Professionals
by Ibrahim Mubarak Al Baalharith and Ahmad Eissa Aboshaiqah
Healthcare 2024, 12(7), 739; https://doi.org/10.3390/healthcare12070739 (registering DOI) - 29 Mar 2024
Abstract
Background: Virtual care adoption accelerated during the COVID-19 pandemic, highlighting the need for healthcare professionals to develop relevant competencies. However, limited evidence exists on the core competencies required for quality virtual care delivery. Objective: This study aimed to identify the critical competencies physicians, [...] Read more.
Background: Virtual care adoption accelerated during the COVID-19 pandemic, highlighting the need for healthcare professionals to develop relevant competencies. However, limited evidence exists on the core competencies required for quality virtual care delivery. Objective: This study aimed to identify the critical competencies physicians, nurses, and other health professionals need for adequate virtual care provision in Saudi Arabia using a Delphi method. Methods: A 3-round Delphi technique was applied with a panel of 42 experts, including policymakers, healthcare professionals, academicians, and telehealth specialists. In Round 1, an open-ended questionnaire elicited competencies needed for virtual care. The competencies were distilled and rated for importance in Rounds 2 and 3 until consensus was achieved. Results: Consensus emerged on 151 competencies across 33 domains. The most prominent domains were communication (15 competencies), professionalism (13), leadership (12), health informatics (5), digital literacy (5), and clinical expertise (11). Full article
(This article belongs to the Special Issue Telehealth and Remote Patient Monitoring)
10 pages, 2400 KiB  
Article
Maternal Care Behavior and Its Consequences in Competition
by Guang-Yun Li, Yu-Chuang Li and Huai Liu
Insects 2024, 15(4), 236; https://doi.org/10.3390/insects15040236 (registering DOI) - 29 Mar 2024
Abstract
Parental care behavior has evolved as a life history strategy to improve reproductive success, particularly in organisms facing challenging environments. However, the variation in maternal care, such as egg-guarding behavior in response to the social environment and the associated ecological consequence of competition, [...] Read more.
Parental care behavior has evolved as a life history strategy to improve reproductive success, particularly in organisms facing challenging environments. However, the variation in maternal care, such as egg-guarding behavior in response to the social environment and the associated ecological consequence of competition, remains largely unknown. This study addresses a gap in current knowledge by examining the plasticity of maternal care behavior in the predatory mite C. eruditus and its impact on offspring survival and intra- and interspecific competition. Our results demonstrated that the reproductive females frequently exhibit egg-guarding behaviors, with enhanced maternal care efforts when the interspecific competitor is present. Egg masses are significantly more vulnerable to predation in the absence of maternal care. Guarding females increased egg survival rates and adversely influenced the survival of both con- and heterospecific competitors, with higher mortality rates being detected. Our findings highlight the ecological significance of maternal care behaviors and suggest that releasing C. eruditus and Neoseiulus cucumeris (Oudemans) together is not recommended for pest management in storage products. Full article
(This article belongs to the Section Insect Behavior and Pathology)
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21 pages, 3057 KiB  
Review
Targeting Siglec–Sialylated MUC1 Immune Axis in Cancer
by Ramya Ayyalasomayajula and Mare Cudic
Cancers 2024, 16(7), 1334; https://doi.org/10.3390/cancers16071334 (registering DOI) - 29 Mar 2024
Abstract
Siglecs play a key role in mediating cell–cell interactions via the recognition of different sialylated glycoconjugates, including tumor-associated MUC1, which can lead to the activation or inhibition of the immune response. The activation occurs through the signaling of Siglecs with the cytoplasmic immunoreceptor [...] Read more.
Siglecs play a key role in mediating cell–cell interactions via the recognition of different sialylated glycoconjugates, including tumor-associated MUC1, which can lead to the activation or inhibition of the immune response. The activation occurs through the signaling of Siglecs with the cytoplasmic immunoreceptor tyrosine-based activation motif (ITAM)-containing proteins, while the inhibition signal is a result of the interaction of intracellular immunoreceptor tyrosine-based inhibition motif (ITIM)-bearing receptors. The interaction of tumor-associated MUC1 sialylated glycans with Siglecs via ITIM motifs decreases antitumor immunity. Consequently, these interactions are expected to play a key role in tumor evasion. Efforts to modulate the response of immune cells by blocking the immune-suppressive effects of inhibitory Siglecs, driving immune-activating Siglecs, and/or altering the synthesis and expression of the sialic acid glycocalyx are new therapeutic strategies deserving further investigation. We will highlight the role of Siglec’s family receptors in immune evasion through interactions with glycan ligands in their natural context, presented on the protein such as MUC1, factors affecting their fine binding specificities, such as the role of multivalency either at the ligand or receptor side, their spatial organization, and finally the current and future therapeutic interventions targeting the Siglec–sialylated MUC1 immune axis in cancer. Full article
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5 pages, 202 KiB  
Editorial
Postharvest Management of Fruits and Vegetables—Series II
by Yoshihiro Imahori and Jinhe Bai
Foods 2024, 13(7), 1049; https://doi.org/10.3390/foods13071049 (registering DOI) - 29 Mar 2024
Abstract
Fruits and vegetables are crucial nutritional sources of carbohydrates, protein, minerals, vitamins, and dietary fiber, offering significant benefits to human health [...] Full article
(This article belongs to the Special Issue Postharvest Management of Fruits and Vegetables Series II)
14 pages, 6833 KiB  
Article
Reducing Automotive Cooling System Complexity through an Adaptive Biomimetic Air Control Valve
by Thomas Thuilot, Moses-Gereon Wullweber, Matthias Fischer, Michael Bennemann and Tobias Seidl
Biomimetics 2024, 9(4), 207; https://doi.org/10.3390/biomimetics9040207 (registering DOI) - 29 Mar 2024
Abstract
Future automotive mobility is predominantly electric. Compared to existing systems, the requirements of subsystems change. Air flow for cooling components is needed predominantly when the car is in rest (i.e., charging) or at slow speeds. So far, actively driven fans consuming power and [...] Read more.
Future automotive mobility is predominantly electric. Compared to existing systems, the requirements of subsystems change. Air flow for cooling components is needed predominantly when the car is in rest (i.e., charging) or at slow speeds. So far, actively driven fans consuming power and generating noise are used in this case. Here we propose a passive adaptive system allowing for convection-driven cooling. The developed system is a highly adaptive flat valve derived from the bordered pit. It was developed through an iterative design process including simulations, both structural and thermodynamic. In hardwoods and conifers, bordered pits enable the challenging transport of vertical fluids by locally limiting damage. Depending on the structure, these can close at sudden pressure changes and take the function of valves. The result of the biomimetic abstraction process is a system-integrative, low-profile valve that is cheap to produce, long-lasting, lightweight, maintenance-free, and noise-free. It allows for the passive switching of air flow generation at the heat exchanger of the cooling between natural convection or an active airstream without the need for complex sensing and control systems. The geometric and material design factors allow for the simple tuning of the valve to the desired switching conditions during the design process. Full article
(This article belongs to the Section Biomimetics of Materials and Structures)
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11 pages, 620 KiB  
Article
Sex Differences in Albumin Quotient and Cerebrospinal Fluid Total Protein Content Do Not Depend on Anthropometric Factors
by Massimiliano Castellazzi, Raffaella Candeloro, Caterina Trevisan, Samantha Permunian, Gaia Buscemi, Sara Ghisellini, Giovanna Negri, Giada Gilli, Caterina Ferri, Tiziana Bellini, Stefano Pizzicotti and Maura Pugliatti
J. Pers. Med. 2024, 14(4), 362; https://doi.org/10.3390/jpm14040362 (registering DOI) - 29 Mar 2024
Abstract
(1) Background: Cerebrospinal fluid (CSF)/serum albumin quotient (QAlb) and CSF total protein (TP) are more elevated in males than females, and this has been hypothesised to be due to anthropometric differences between the sexes. This study aimed to investigate QAlb and CSF TP [...] Read more.
(1) Background: Cerebrospinal fluid (CSF)/serum albumin quotient (QAlb) and CSF total protein (TP) are more elevated in males than females, and this has been hypothesised to be due to anthropometric differences between the sexes. This study aimed to investigate QAlb and CSF TP as a function of body height, weight, and body mass index (BMI). (2) Methods: A total of 207 patients were included in the study and analysed blinded to clinical diagnosis. (3) Results: Multivariable linear regressions were run to predict log-transformed Qalb and log-transformed CSF TP value from age, sex, weight, and height (first model) or from age, sex, and BMI (second model). In both models, age (β = 0.004, 95% CI = 0.002 to 0.006) and sex (β = −0.095, 95% CI = −0.169 to −0.021, and β = −0.135, 95% CI = −0.191 to −0.079) were significant predictors for QAlb, but weight, height, and BMI were not. Similarly, age (β = 0.004, 95% CI = 0.003 to 0.006) and sex (β = −0.077, 95% CI = −0.142 to −0.013, and β = −0.109, 95% CI = −0.157 to −0.060) were significant predictors for CSF TP, while anthropometric characteristics were not. No differences in QAlb and CSF TP were found when grouping males and females by BMI status. (4) Conclusions: Our data suggest that anthropometric characteristics could not explain the sex-related differences in QAlb and CSF TP. Full article
(This article belongs to the Special Issue Sex and Gender-Related Issues in the Era of Personalized Medicine)
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15 pages, 2078 KiB  
Article
Synergistic Antibacterial Effects of Gallate Containing Compounds with Silver Nanoparticles in Gallate Crossed Linked PVA Hydrogel Films
by John Jackson and Claudia Helena Dietrich
Antibiotics 2024, 13(4), 312; https://doi.org/10.3390/antibiotics13040312 (registering DOI) - 29 Mar 2024
Abstract
Currently available silver-based antiseptic wound dressings have limited patient effectiveness. There exists a need for wound dressings that behave as comfortable degradable hydrogels with a strong antibiotic potential. The objectives of this project were to investigate the combined use of gallates (either epi [...] Read more.
Currently available silver-based antiseptic wound dressings have limited patient effectiveness. There exists a need for wound dressings that behave as comfortable degradable hydrogels with a strong antibiotic potential. The objectives of this project were to investigate the combined use of gallates (either epi gallo catechin gallate (EGCG), Tannic acid, or Quercetin) as both PVA crosslinking agents and as potential synergistic antibiotics in combination with silver nanoparticles. Crosslinking was assessed gravimetrically, silver and gallate release was measured using inductively coupled plasma and HPLC methods, respectively. Synergy was measured using 96-well plate FICI methods and in-gel antibacterial effects were measured using planktonic CFU assays. All gallates crosslinked PVA with optimal extended swelling obtained using EGCG or Quercetin at 14% loadings (100 mg in 500 mg PVA with glycerol). All three gallates were synergistic in combination with silver nanoparticles against both gram-positive and -negative bacteria. In PVA hydrogel films, silver nanoparticles with EGCG or Quercetin more effectively inhibited bacterial growth in CFU counts over 24 h as compared to films containing single agents. These biocompatible natural-product antibiotics, EGCG or Quercetin, may play a dual role of providing stable PVA hydrogel films and a powerful synergistic antibiotic effect in combination with silver nanoparticles. Full article
(This article belongs to the Special Issue Nanocarriers-Based Antimicrobial Drug Delivery)
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16 pages, 1411 KiB  
Article
Valorization of Wood Residues from Vegetation Suppression during Wind Energy Plant Implementation and Its Potential for Renewable Phenolic Compounds through Flash Pyrolysis: A Case Study in Northeast Brazil’s Semi-Arid Region
by Marcela Cristina Pereira dos Santos Almeida, Janduir Egito da Silva, Willame Gomes da Silva Batista, José Luiz Francisco Alves, Dulce Maria de Araújo Melo, Alexandre Santos Pimenta and Renata Martins Braga
Forests 2024, 15(4), 621; https://doi.org/10.3390/f15040621 (registering DOI) - 29 Mar 2024
Abstract
In the past few years, wind power has become a viable alternative in Brazil to diversify the energy mix and mitigate pollutant emissions from fossil fuels. Significant wind energy generation potential is inherent in the Brazilian Northeast state of Rio Grande do Norte, [...] Read more.
In the past few years, wind power has become a viable alternative in Brazil to diversify the energy mix and mitigate pollutant emissions from fossil fuels. Significant wind energy generation potential is inherent in the Brazilian Northeast state of Rio Grande do Norte, due to prevailing strong winds along the coastline and elevated regions. However, clean and renewable wind energy may lead to potential biodiversity impacts, including the removal of native vegetation during plant construction and operation. This case study explores the flash pyrolysis-based valorization of three commonly suppressed species, namely Cenostigma pyramidale (CP), Commiphora leptophloeos (CL), and Aspidosperma pyrifolium (AP), in a wind farm situated within the Mato Grande region of Rio Grande do Norte State. The study centers on determining their bioenergy-related properties and assessing their potential for producing phenolic-rich bio-oil. The investigation of three wood residues as potential sources of high-value chemicals, specifically phenolic compounds, was conducted using a micro-furnace type temperature programmable pyrolyzer combined with gas chromatography/mass spectrometry (Py–GC/MS setup). The range of higher heating values observed for three wood residues was 17.5–18.4 MJ kg−1, with the highest value attributed to AP wood residue. The bulk density ranged from 126.5 to 268.7 kg m−3, while ash content, volatile matter content, fixed carbon content, and lignin content were within the respective ranges of 0.8–2.9 wt.%, 78.5–89.6 wt.%, 2.6–9.5 wt.%, and 19.1–30.6 wt.%. Although the energy-related properties signifying the potential value of three wood residues as energy resources are evident, their applicability in the bioenergy sector can be expanded via pelleting or briquetting. Yields of phenolic compounds exceeding 40% from the volatile pyrolysis products of CL and AP wood residues at 500 °C make them favorable for phenolic-rich bio-oil production. The findings of this study endorse the utilization of wood residues resulting from vegetation suppression during the installation of wind energy plants as potential feedstocks for producing bioenergy and sustainable phenolic compounds. This presents a solution for addressing a regional environmental concern following the principles of green chemistry. Full article
(This article belongs to the Special Issue Bioenergy from Wood: Sustainable Production in the World)
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15 pages, 258 KiB  
Article
Is Obesity a Cause for Shame? Weight Bias and Stigma among Physicians, Dietitians, and Other Healthcare Professionals in Poland—A Cross-Sectional Study
by Alicja Baska, Karolina ?wider, Wojciech Stefan Zgliczyński, Karolina K?oda, Agnieszka Mastalerz-Migas and Mateusz Babicki
Nutrients 2024, 16(7), 999; https://doi.org/10.3390/nu16070999 (registering DOI) - 29 Mar 2024
Abstract
Weight bias and weight stigma pose significant challenges in healthcare, particularly affecting obesity management practices and patient care quality. Our study evaluates their prevalence and impact among healthcare professionals in Poland. Using the Fat Phobia Scale and custom questions, we surveyed 686 professionals [...] Read more.
Weight bias and weight stigma pose significant challenges in healthcare, particularly affecting obesity management practices and patient care quality. Our study evaluates their prevalence and impact among healthcare professionals in Poland. Using the Fat Phobia Scale and custom questions, we surveyed 686 professionals via Computer-Assisted Web Interview (CAWI). Results reveal a moderate level of explicit weight bias (mean score: 3.60 ± 0.57), with significant variations across professional groups: physicians (3.70 ± 0.48), dietitians (3.51 ± 0.48), and others (3.44 ± 0.77). Common feelings towards individuals with obesity include willingness to help (57.0%) and compassion (37.8%), yet 29.9% perceive obesity as shameful. The results also vary depending on the respondent’s sex or BMI. These findings underscore the need for evidence-based interventions to mitigate weight stigma and enhance understanding of obesity among healthcare professionals. Full article
(This article belongs to the Special Issue Public Health, Nutritional Behavior and Nutritional Status)
12 pages, 1329 KiB  
Article
Enhancing the Fermentation Process in Biogas Production from Animal and Plant Waste Substrates in the Southeastern Region of Bulgaria
by Angel Terziev, Penka Zlateva and Martin Ivanov
Fermentation 2024, 10(4), 187; https://doi.org/10.3390/fermentation10040187 (registering DOI) - 29 Mar 2024
Abstract
Annually, a huge amount of waste from plant biomass and animal manure is produced from agriculture and animal farming. Many studies provide information on the biomethane potential of agricultural and livestock wastes, but only a few studies have investigated the application of the [...] Read more.
Annually, a huge amount of waste from plant biomass and animal manure is produced from agriculture and animal farming. Many studies provide information on the biomethane potential of agricultural and livestock wastes, but only a few studies have investigated the application of the substrates in combination. The objective of the study is to enhance the fermentation process in the digester for biogas production, obtained from animal and plant waste substrates. In four batch processes for three months, the temperatures and the residence time of the substrates in the fermenter were analyzed. Simultaneously, electricity and thermal energy were produced via cogeneration units, which were exported to the public grid and city heating network. The plant substrate is a silage mixture of corn and wheat waste. The animal substrate is a mixture of beef and pig manure. Animal and vegetable waste raw materials are collected and transported to the site, located in the region of southeastern Bulgaria. The total annual consumption of animal and plant waste is 17,971 t/year. The enhancement of the process leads to the production of 1,506,000 Nm3 CH4/a of methane, the generation of which requires 299.63 MWh/a of electricity and 649.09 MWh/a thermal energy. Full article
(This article belongs to the Special Issue Biofuels Production and Processing Technology: 3rd Edition)
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13 pages, 1409 KiB  
Article
Improving Blood Vessel Segmentation and Depth Estimation in Laser Speckle Images Using Deep Learning
by Eduardo Morales-Vargas, Hayde Peregrina-Barreto, Rita Q. Fuentes-Aguilar, Juan Pablo Padilla-Martinez, Wendy Argelia Garcia-Suastegui and Julio C. Ramirez-San-Juan
Information 2024, 15(4), 185; https://doi.org/10.3390/info15040185 (registering DOI) - 29 Mar 2024
Abstract
Microvasculature analysis is an important task in the medical field due to its various applications. It has been used for the diagnosis and threat of diseases in fields such as ophthalmology, dermatology, and neurology by measuring relative blood flow or blood vessel morphological [...] Read more.
Microvasculature analysis is an important task in the medical field due to its various applications. It has been used for the diagnosis and threat of diseases in fields such as ophthalmology, dermatology, and neurology by measuring relative blood flow or blood vessel morphological properties. However, light scattering at the periphery of the blood vessel causes a decrease in contrast around the vessel borders and an increase in the noise of the image, making the localization of blood vessels a challenging task. Therefore, this work proposes integrating known information from the experimental setup into a deep learning architecture with multiple inputs to improve the generalization of a computational model for the segmentation of blood vessels and depth estimation in a single inference step. The proposed R-UNET + ET + LA obtained an intersection over union of 0.944 ± 0.065 and 0.812 ± 0.080 in the classification task for validation (in vitro) and test sets (in vivo), respectively, and a root mean squared error of 0.0085 ± 0.0275 μm in the depth estimation. This approach improves the generalization of current solutions by pre-training with in vitro data and adding information from the experimental setup. Additionally, the method can infer the depth of a blood vessel pixel by pixel instead of in regions as the current state of the art does. Full article
(This article belongs to the Special Issue Applications of Deep Learning in Bioinformatics and Image Processing)
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12 pages, 2473 KiB  
Article
Study of the Use of Gas Diffusion Anode with Various Cathodes (Cu-Ag, Ni-Co, and Cu-B Alloys) in a Microbial Fuel Cell
by Pawe? P. W?odarczyk and Barbara W?odarczyk
Energies 2024, 17(7), 1636; https://doi.org/10.3390/en17071636 (registering DOI) - 29 Mar 2024
Abstract
Advancing microbial fuel cell (MFC) technologies appears to be a crucial direction in bolstering wastewater treatment efforts. It ensures both energy recovery (bioelectricity production) and wastewater pre-treatment. One of the problems in the widespread use of MFCs is the generation of a small [...] Read more.
Advancing microbial fuel cell (MFC) technologies appears to be a crucial direction in bolstering wastewater treatment efforts. It ensures both energy recovery (bioelectricity production) and wastewater pre-treatment. One of the problems in the widespread use of MFCs is the generation of a small amount of electricity. Hence, a pivotal concern revolves around enhancing the efficiency of this process. One avenue of investigation in this realm involves the selection of electrode materials. In this research, a carbon-based gas diffusion electrode (GDE) was used as the anode of MFC. Whereas for the cathode, a copper mesh with various catalysts (Cu-B, Ni-Co, and Cu-Ag) was used. This research was conducted in glass MFCs with the sintered glass acting as a chamber separator. This research was conducted for various electrode systems (GDE/Cu-Ag, GDE/Ni-Co, and GDE/Cu-B). This study analyzed both the electrical parameters and chemical oxygen demand (COD) reduction time. In each case (for each electrode system), bioelectricity production was achieved. This work shows that when GDE is used as the anode and Cu-B, Ni-Co and Cu-Ag alloys as the cathode, the most efficient system is the GDE/Cu-Ag system. It ensures the fastest start-up, the highest power density, and the shortest COD reduction time. Full article
(This article belongs to the Section A4: Bio-Energy)
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14 pages, 2481 KiB  
Article
Chemical Recalcitrance Rather Than Soil Microbial Community Determined Short-Term Biochar Stability in a Poplar Plantation Soil
by Fangchao Zhang, Weiwei Lu and Fengjie Jin
Forests 2024, 15(4), 622; https://doi.org/10.3390/f15040622 (registering DOI) - 29 Mar 2024
Abstract
The stability of biochar is fundamental to its soil carbon (C) sequestration potential. The relative importance of chemical recalcitrance and the soil microbial community on biochar stability is still unclear. To unveil the question, we conducted a 60-day incubation to explore the stability [...] Read more.
The stability of biochar is fundamental to its soil carbon (C) sequestration potential. The relative importance of chemical recalcitrance and the soil microbial community on biochar stability is still unclear. To unveil the question, we conducted a 60-day incubation to explore the stability of two rice-straw-derived biochars pyrolyzed at 300 and 500 °C (denoted as BS300 and BS500), as well as the relative contribution of the soil microbial community and biochar chemical recalcitrance to biochar stability in a poplar plantation soil. Biochar-derived cumulative carbon dioxide (CO2) emission was estimated to be 41.3 and 6.80 mg C kg−1, accounting for 0.73 and 0.11% of the amended biochar-derived organic C (OC) in BS300 and BS500 treatments, respectively. The mean retention time (MRT) estimated by double-exponential model fitting was 49.4 years for BS300 and 231 years for BS500. Compared to control, BS300 and BS500 decreased β-D-glucosidase activity by 20.9 and 18.0%, while they decreased phenol oxidase activity by 31.8 and 18.9%, respectively. Furthermore, BS300 increased the soil microbial metabolic quotient (qCO2) by 155%, but BS500 decreased it by 13.4%. In addition, BS300 resulted in a 520% higher biochar-derived hot-water-extractable OC than BS500. Partial least-squares path modeling (PLSPM) showed that the path efficients of biochar’s chemical recalcitrance and microbial qCO2 were 0.52 and 0.25, respectively, and that of the soil microbial activity is neglected. We conclude from this short-term study that chemical recalcitrance imposed a greater effect than soil microbial community on biochar stability. Full article
(This article belongs to the Section Forest Soil)
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14 pages, 875 KiB  
Review
The Microbial Community of Natural Whey Starter: Why Is It a Driver for the Production of the Most Famous Italian Long-Ripened Cheeses?
by Erasmo Neviani, Alessia Levante and Monica Gatti
Fermentation 2024, 10(4), 186; https://doi.org/10.3390/fermentation10040186 (registering DOI) - 29 Mar 2024
Abstract
The remarkable global diversity in long-ripened cheese production can be attributed to the adaptability of the cheese microbiota. Most cheese types involve intricate microbial ecosystems, primarily represented by lactic acid bacteria (LAB). The present study aims to review the microbial community’s diversity in [...] Read more.
The remarkable global diversity in long-ripened cheese production can be attributed to the adaptability of the cheese microbiota. Most cheese types involve intricate microbial ecosystems, primarily represented by lactic acid bacteria (LAB). The present study aims to review the microbial community’s diversity in dairy fermentation processes, focusing on two famous Italian cheeses, Grana Padano and Parmigiano Reggiano, produced using natural whey starter (NWS). NWS, created by retaining whey from the previous day’s cheese batches, forms a microbiological connection between daily cheese productions. Through this technique, a dynamic microbiota colonizes the curd and influences cheese ripening. The back-slopping method in NWS preparation ensures the survival of diverse biotypes, providing a complex microbial community in which interactions among microorganisms are critical to ensuring its technological functionality. As highlighted in this review, the presence of microbial cells alone does not guarantee technological relevance. Critical microorganisms can grow and colonize the curd and cheese. This complexity enables NWS to adapt to artisanal production technologies while considering variations in raw milk microbiota, inhibitory compounds, and manufacturing conditions. This critical review aims to discuss NWS as a key factor in cheese making, considering microbial communities’ ability to evolve under different selective pressures and biotic and abiotic stresses. Full article
(This article belongs to the Special Issue Development and Application of Starter Cultures)
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20 pages, 7029 KiB  
Article
Protective Effect of Curcumin on D-Galactose-Induced Senescence and Oxidative Stress in LLC-PK1 and HK-2 Cells
by Semiramis Stephania García-Trejo, Tania Gómez-Sierra, Dianelena Eugenio-Pérez, Omar Noel Medina-Campos and José Pedraza-Chaverri
Antioxidants 2024, 13(4), 415; https://doi.org/10.3390/antiox13040415 (registering DOI) - 29 Mar 2024
Abstract
D-galactose has been widely used as an inducer of cellular senescence and pathophysiological processes related to aging because it induces oxidative stress. On the other hand, the consumption of antioxidants such as curcumin can be an effective strategy to prevent phenotypes related to [...] Read more.
D-galactose has been widely used as an inducer of cellular senescence and pathophysiological processes related to aging because it induces oxidative stress. On the other hand, the consumption of antioxidants such as curcumin can be an effective strategy to prevent phenotypes related to the enhanced production of reactive oxygen species (ROS), such as aging and senescence. This study aimed to evaluate the potential protective effect of curcumin on senescence and oxidative stress and endoplasmic reticulum stress induced by D-galactose treatment in Lilly Laboratories Culture-Porcine Kidney 1 (LLC-PK1) and human kidney 2 (HK-2) proximal tubule cell lines from pig and human, respectively. For senescence induction, cells were treated with 300 mM D-galactose for 120 h and, to evaluate the protective effect of the antioxidant, cells were treated with 5 µM curcumin for 24 h and subsequently treated with curcumin + D-galactose for 120 h. In LLC-PK1 cells, curcumin treatment decreased by 20% the number of cells positive for senescence-associated (SA)-β-D-galactosidase staining and by 25% the expression of 8-hydroxy-2′-deoxyguanosine (8-OHdG) and increased by 40% lamin B1 expression. In HK-2 cells, curcumin treatment increased by 60% the expression of proliferating cell nuclear antigen (PCNA, 50% Klotho levels, and 175% catalase activity. In both cell lines, this antioxidant decreased the production of ROS (20% decrease for LLC-PK1 and 10 to 20% for HK-2). These data suggest that curcumin treatment has a moderate protective effect on D-galactose-induced senescence in LLC-PK1 and HK-2 cells. Full article
(This article belongs to the Special Issue Regulatory Effects of Curcumin)
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