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18 pages, 829 KiB  
Article
Power Flow Regulation Effect and Parameter Design Method of Phase-Shifting Transformer
by Weigang Jin, Hangya Liu, Weizhe Zhang and Jiaxin Yuan
Energies 2024, 17(7), 1622; https://doi.org/10.3390/en17071622 (registering DOI) - 28 Mar 2024
Abstract
In the context of carbon peaking and carbon neutrality goals, the integration of a large number of renewable energy sources may trigger significant tidal changes, leading to transmission congestion, wind and light abandonment, power oscillation, voltage and frequency fluctuations, etc. A phase-shifting transformer [...] Read more.
In the context of carbon peaking and carbon neutrality goals, the integration of a large number of renewable energy sources may trigger significant tidal changes, leading to transmission congestion, wind and light abandonment, power oscillation, voltage and frequency fluctuations, etc. A phase-shifting transformer is a cost-effective and reliable power flow control equipment, but its key parameters lack systematic design methods. Based on the equivalent model of phase-shifting transformers, detailed design principles and methods have been proposed, and the configuration method of winding turns has been improved to facilitate calculation and control strategy formulation. The regulation effect of the phase-shifting transformer designed through simulation of power flow regulation in a typical 500 kV network is simulated. By adjusting the phase-shifting transformer, the power flow of heavy load lines can be transferred to light load lines, optimizing the power flow distribution and improving the transmission capacity of the cross-section. Full article
45 pages, 1119 KiB  
Review
CC Chemokine Family Members’ Modulation as a Novel Approach for Treating Central Nervous System and Peripheral Nervous System Injury—A Review of Clinical and Experimental Findings
by Agata Ciechanowska and Joanna Mika
Int. J. Mol. Sci. 2024, 25(7), 3788; https://doi.org/10.3390/ijms25073788 (registering DOI) - 28 Mar 2024
Abstract
Despite significant progress in modern medicine and pharmacology, damage to the nervous system with various etiologies still poses a challenge to doctors and scientists. Injuries lead to neuroimmunological changes in the central nervous system (CNS), which may result in both secondary damage and [...] Read more.
Despite significant progress in modern medicine and pharmacology, damage to the nervous system with various etiologies still poses a challenge to doctors and scientists. Injuries lead to neuroimmunological changes in the central nervous system (CNS), which may result in both secondary damage and the development of tactile and thermal hypersensitivity. In our review, based on the analysis of many experimental and clinical studies, we indicate that the mechanisms occurring both at the level of the brain after direct damage and at the level of the spinal cord after peripheral nerve damage have a common immunological basis. This suggests that there are opportunities for similar pharmacological therapeutic interventions in the damage of various etiologies. Experimental data indicate that after CNS/PNS damage, the levels of 16 among the 28 CC-family chemokines, i.e., CCL1, CCL2, CCL3, CCL4, CCL5, CCL6, CCL7, CCL8, CCL9, CCL11, CCL12, CCL17, CCL19, CCL20, CCL21, and CCL22, increase in the brain and/or spinal cord and have strong proinflammatory and/or pronociceptive effects. According to the available literature data, further investigation is still needed for understanding the role of the remaining chemokines, especially six of them which were found in humans but not in mice/rats, i.e., CCL13, CCL14, CCL15, CCL16, CCL18, and CCL23. Over the past several years, the results of studies in which available pharmacological tools were used indicated that blocking individual receptors, e.g., CCR1 (J113863 and BX513), CCR2 (RS504393, CCX872, INCB3344, and AZ889), CCR3 (SB328437), CCR4 (C021 and AZD-2098), and CCR5 (maraviroc, AZD-5672, and TAK-220), has beneficial effects after damage to both the CNS and PNS. Recently, experimental data have proved that blockades exerted by double antagonists CCR1/3 (UCB 35625) and CCR2/5 (cenicriviroc) have very good anti-inflammatory and antinociceptive effects. In addition, both single (J113863, RS504393, SB328437, C021, and maraviroc) and dual (cenicriviroc) chemokine receptor antagonists enhanced the analgesic effect of opioid drugs. This review will display the evidence that a multidirectional strategy based on the modulation of neuronal–glial–immune interactions can significantly improve the health of patients after CNS and PNS damage by changing the activity of chemokines belonging to the CC family. Moreover, in the case of pain, the combined administration of such antagonists with opioid drugs could reduce therapeutic doses and minimize the risk of complications. Full article
(This article belongs to the Special Issue The Multiple Mechanisms Underlying Neuropathic Pain (III))
19 pages, 3420 KiB  
Article
Blood-Based DNA Methylation Analysis by Multiplexed OBBPA-ddPCR to Verify Indications for Prostate Biopsies in Suspected Prostate Cancer Patients
by Markus Friedemann, Carsten Jandeck, Lars Tautz, Katharina Gutewort, Lisa von Rein, Olga Sukocheva, Susanne Fuessel and Mario Menschikowski
Cancers 2024, 16(7), 1324; https://doi.org/10.3390/cancers16071324 (registering DOI) - 28 Mar 2024
Abstract
Current prostate carcinoma (PCa) biomarkers, including total prostate-specific antigen (tPSA), have unsatisfactory diagnostic sensitivity and specificity resulting in overdiagnosis and overtreatment. Previously, we described an optimised bias-based preamplification–digital droplet PCR (OBBPA-ddPCR) technique, which detects tumour DNA in blood-derived cell-free DNA (cfDNA) of cancer [...] Read more.
Current prostate carcinoma (PCa) biomarkers, including total prostate-specific antigen (tPSA), have unsatisfactory diagnostic sensitivity and specificity resulting in overdiagnosis and overtreatment. Previously, we described an optimised bias-based preamplification–digital droplet PCR (OBBPA-ddPCR) technique, which detects tumour DNA in blood-derived cell-free DNA (cfDNA) of cancer patients. The current study investigated the performance of newly developed OBBPA-ddPCR-based biomarkers. Blood plasma samples from healthy individuals (n = 90, controls) and PCa (n = 39) and benign prostatic hyperplasia patients (BPH, n = 40) were analysed. PCa and BPH patients had tPSA values within a diagnostic grey area of 2–15 ng/mL, for whom further diagnostic validation is most crucial. Methylation levels of biomarkers RASSF1A, MIR129-2, NRIP3, and SOX8 were found significantly increased in PCa patients compared to controls. By combining classical PCa risk factors (percentage of free PSA compared to tPSA (QfPSA) and patient’s age) with cfDNA-based biomarkers, we developed PCa risk scores with improved sensitivity and specificity compared to established tPSA and QfPSA single-marker analyses. The diagnostic specificity was increased to 70% with 100% sensitivity for clinically significant PCa patients. Thus, prostate biopsies could be avoided for 28 out of 40 BPH patients. In conclusion, the newly developed risk scores may help to confirm the clinical decision and prevent unnecessary prostate biopsy. Full article
(This article belongs to the Section Cancer Pathophysiology)
23 pages, 4184 KiB  
Article
Spatial Dynamics and Social Order in Traditional Towns of Saudi Arabia’s Nadji Region: The Role of Neighborhood Clustering in Urban Morphology and Decision-Making Processes
by Mohammed Mashary Alnaim and Emad Noaime
Sustainability 2024, 16(7), 2830; https://doi.org/10.3390/su16072830 (registering DOI) - 28 Mar 2024
Abstract
This study explores how the layout of neighborhoods in traditional settlements of Saudi Arabia’s Najdi region influence social interactions and urban planning decisions. The study uses a multidisciplinary approach that includes urban morphology, architectural phenomenology, and sociological study methods to investigate the relationships [...] Read more.
This study explores how the layout of neighborhoods in traditional settlements of Saudi Arabia’s Najdi region influence social interactions and urban planning decisions. The study uses a multidisciplinary approach that includes urban morphology, architectural phenomenology, and sociological study methods to investigate the relationships between spatial organization and decision-making processes on both the macro and micro levels of decision-making. The purpose is to look at how collective action decision-making processes affect the urban fabric and how social norms influence spatial organization at different levels. The study applies case study and spatial analysis approaches to investigate how the traditional settlements’ spatial structure promotes peace among the inhabitants while also sustaining cultural traditions. The qualitative approach investigates how spatial arrangements influence behaviors, developing a better understanding of how residents interact with their surroundings. According to the study’s findings, these spatial layouts sustain customs and assist communities in adapting to environmental changes by retaining cultural activities. The study identifies the significance of balancing development with the retention of important traditional values in the implementation of long-term urban conservation plans. Traditional Najdi towns can serve as urban design examples, emphasizing the need to acknowledge the distinct value of vernacular architecture in modern urban development while also fostering social cohesion. Full article
(This article belongs to the Special Issue Architecture, Urban Space and Heritage in the Digital Age)
20 pages, 354 KiB  
Article
UK Dog Owners’ Pre-Acquisition Information- and Advice-Seeking: A Mixed Methods Study
by Rebecca Mead, Katrina E. Holland, Rachel A. Casey, Melissa M. Upjohn and Robert M. Christley
Animals 2024, 14(7), 1033; https://doi.org/10.3390/ani14071033 (registering DOI) - 28 Mar 2024
Abstract
Dogs are the most common pet animal species in the UK. Little is known about information and advice gathering within the process of dog acquisition, nor what pre-acquisition research encompasses. This study aimed to better understand the preparatory research undertaken by prospective dog [...] Read more.
Dogs are the most common pet animal species in the UK. Little is known about information and advice gathering within the process of dog acquisition, nor what pre-acquisition research encompasses. This study aimed to better understand the preparatory research undertaken by prospective dog owners in the UK. A 2019 online survey collected quantitative and qualitative data about dog acquisition. Analyses within this study focused on a subset of these current (n = 4381) and potential (n = 2350) owners who had undertaken research, or who reported planning to do so, before acquiring a dog. Additional qualitative data were collected through semi-structured interviews with current (n = 24) and potential (n = 8) dog owners. Among those current owners who had sought information or advice, websites were the most common source of information (76%), followed by family and friends (70%), and online forums (51%). Similar patterns were seen among potential owners. Qualitative data revealed that information was commonly sought on the following: general information about dogs; information about breeds or types of dogs; owner requirements and dog suitability; aspects of dog ownership; and how to source a dog. The majority of prospective owners stated that they had found all the information they wanted (96% of current owners and 90% of potential owners) but some respondents found conflicting advice from different sources and did not know which sources to trust. Our study shows that, for some prospective owners, research into various aspects of dog acquisition and ownership is important. Knowing where to look for correct and unbiased advice, particularly online, is particularly important. Understanding the pre-acquisition research that owners undertake, including the sources they use, information they are interested in finding, and the challenges they face, is of interest to animal welfare and veterinary organisations and those involved in rehoming and selling dogs. This information may help inform interventions aimed at improving the knowledge and decisions made by potential dog owners. Full article
19 pages, 2106 KiB  
Article
Assessment of Silver Nanoparticles Derived from Brown Algae Sargassum vulgare: Insight into Antioxidants, Anticancer, Antibacterial and Hepatoprotective Effect
by Ragaa A. Hamouda and Ebtehail S. Aljohani
Mar. Drugs 2024, 22(4), 154; https://doi.org/10.3390/md22040154 (registering DOI) - 28 Mar 2024
Abstract
Algae are used as safe materials to fabricate novel nanoparticles to treat some diseases. Marine brown alga Sargassum vulgare are used to fabricate silver nanoparticles (Sv/Ag-NPs). The characterization of Sv/Ag-NPs was determined by TEM, EDX, Zeta potential, XRD, and UV spectroscopy. The Sv/Ag-NPs [...] Read more.
Algae are used as safe materials to fabricate novel nanoparticles to treat some diseases. Marine brown alga Sargassum vulgare are used to fabricate silver nanoparticles (Sv/Ag-NPs). The characterization of Sv/Ag-NPs was determined by TEM, EDX, Zeta potential, XRD, and UV spectroscopy. The Sv/Ag-NPs were investigated as antioxidant, anticancer, and antibacterial activities against Gram-positive bacteria Bacillus mojavensis PP400982, Staphylococcus caprae PP401704, Staphylococcus capitis PP402689, and Staphylococcus epidermidis PP403851. The activity of the Sv/Ag-NPs was evaluated as hepatoprotective in vitro in comparison with silymarin. The UV–visible spectrum of Sv/Ag-NPs appeared at 442 nm; the size of Sv/Ag-NPs is in range between 6.90 to 16.97 nm, and spherical in shape. Different concentrations of Sv/Ag-NPs possessed antioxidant, anticancer activities against (HepG-2), colon carcinoma (HCT-116), cervical carcinoma (HeLa), and prostate carcinoma (PC-3) with IC50 50.46, 45.84, 78.42, and 100.39 µg/mL, respectively. The Sv/Ag-NPs induced the cell viability of Hep G2 cells and hepatocytes treated with carbon tetrachloride. The Sv/Ag-NPs exhibited antibacterial activities against Staphylococcus caprae PP401704, Staphylococcus capitis PP402689, and Staphylococcus epidermidis PP403851. This study strongly suggests the silver nanoparticles derived from Sargassum vulgare showed potential hepato-protective effect against carbon tetrachloride-induced liver cells, and could be used as anticancer and antibacterial activities. Full article
(This article belongs to the Special Issue Nanoparticle Synthesis with Marine Substances 2.0)
14 pages, 1984 KiB  
Article
Image Filtering to Improve Maize Tassel Detection Accuracy Using Machine Learning Algorithms
by Eric Rodene, Gayara Demini Fernando, Ved Piyush, Yufeng Ge, James C. Schnable, Souparno Ghosh and Jinliang Yang
Sensors 2024, 24(7), 2172; https://doi.org/10.3390/s24072172 (registering DOI) - 28 Mar 2024
Abstract
Unmanned aerial vehicle (UAV)-based imagery has become widely used to collect time-series agronomic data, which are then incorporated into plant breeding programs to enhance crop improvements. To make efficient analysis possible, in this study, by leveraging an aerial photography dataset for a field [...] Read more.
Unmanned aerial vehicle (UAV)-based imagery has become widely used to collect time-series agronomic data, which are then incorporated into plant breeding programs to enhance crop improvements. To make efficient analysis possible, in this study, by leveraging an aerial photography dataset for a field trial of 233 different inbred lines from the maize diversity panel, we developed machine learning methods for obtaining automated tassel counts at the plot level. We employed both an object-based counting-by-detection (CBD) approach and a density-based counting-by-regression (CBR) approach. Using an image segmentation method that removes most of the pixels not associated with the plant tassels, the results showed a dramatic improvement in the accuracy of object-based (CBD) detection, with the cross-validation prediction accuracy (r2) peaking at 0.7033 on a detector trained with images with a filter threshold of 90. The CBR approach showed the greatest accuracy when using unfiltered images, with a mean absolute error (MAE) of 7.99. However, when using bootstrapping, images filtered at a threshold of 90 showed a slightly better MAE (8.65) than the unfiltered images (8.90). These methods will allow for accurate estimates of flowering-related traits and help to make breeding decisions for crop improvement. Full article
(This article belongs to the Special Issue AI-Driven Sensing for Image Processing and Recognition)
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48 pages, 8619 KiB  
Review
Global Occurrence, Geology and Characteristics of Hydrothermal-Origin Kaolin Deposits
by ?mer I??k Ece and Hatice ?nal Ercan
Minerals 2024, 14(4), 353; https://doi.org/10.3390/min14040353 (registering DOI) - 28 Mar 2024
Abstract
Kaolin-group minerals occur in nature as the result of high-sulfidation acid sulfate, sulfur-poor HCl-, HF- and H2CO3-rich acidic fluid-related hydrothermal alterations and in situ geochemical weathering. These minerals possess different crystallographic and chemical properties that determine their application areas, [...] Read more.
Kaolin-group minerals occur in nature as the result of high-sulfidation acid sulfate, sulfur-poor HCl-, HF- and H2CO3-rich acidic fluid-related hydrothermal alterations and in situ geochemical weathering. These minerals possess different crystallographic and chemical properties that determine their application areas, mainly in the ceramic and paper industries, and as nanocomposite materials. The physicochemical properties of hydrothermal kaolin deposits are the result of the type of parent rock, the effect of the regional tectonism-associated magmatism, and the chemical features of hydrothermal fluids that interact with the deep basement rocks. However, understanding these geothermal systems is one of the most challenging issues due to the rich mineralogical assemblages, complex geochemistry and isotopic data of hydrothermal alteration zones. This study evaluates the formation of hydrothermal-origin kaolin-group minerals by considering their characteristics of hydrothermal alteration, isotopic compositions and differences in characteristic properties of low- and high-sulfidation occurrences; this paper also addresses mineralogical and structural differences between hypogene and supergene kaolin formations, and kaolin–alunite–pyrophyllite association, and it provides examples of worldwide occurrences. The study of the mineralogical assemblages, geochemistry and isotopic data of the hydrothermal alteration zones is one of the most challenging subjects in terms of gaining a detailed understanding of the geothermal systems. Silicification processes are subsequent to late-stage alteration after the completion of kaolinization processes, erasing existing hydrothermal mineralogical and geochemical traces and making interpretation difficult. In the early stages involving magmatic–hydrothermal-origin acidic geothermal fluids, the latter comes from the disproportionation of SO2 (+H2O) and H2S oxidation to H2SO4 in hydrothermal environments. In the later stages, due to spatial and temporal changes over time in the chemistry of geothermal fluids, the system comes to have a more alkali–chloride composition, with neutral pH waters frequently saturated with amorphous silica which characteristically precipitate as siliceous sinter deposits containing large amounts of opal-A. Full article
(This article belongs to the Section Mineral Deposits)
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20 pages, 1256 KiB  
Article
The Dual Role of Neutrophil Extracellular Traps (NETs) in Sepsis and Ischemia-Reperfusion Injury: Comparative Analysis across Murine Models
by Antonia Kiwit, Yuqing Lu, Moritz Lenz, Jasmin Knopf, Christoph Mohr, Yannick Ledermann, Michaela Klinke-Petrowsky, Laia Pagerols Raluy, Konrad Reinshagen, Martin Herrmann, Michael Boettcher and Julia Elrod
Int. J. Mol. Sci. 2024, 25(7), 3787; https://doi.org/10.3390/ijms25073787 (registering DOI) - 28 Mar 2024
Abstract
A better understanding of the function of neutrophil extracellular traps (NETs) may facilitate the development of interventions for sepsis. The study aims to investigate the formation and degradation of NETs in three murine sepsis models and to analyze the production of reactive oxygen [...] Read more.
A better understanding of the function of neutrophil extracellular traps (NETs) may facilitate the development of interventions for sepsis. The study aims to investigate the formation and degradation of NETs in three murine sepsis models and to analyze the production of reactive oxygen species (ROS) during NET formation. Murine sepsis was induced by midgut volvulus (720° for 15 min), cecal ligation and puncture (CLP), or the application of lipopolysaccharide (LPS) (10 mg/kg body weight i.p.). NET formation and degradation was modulated using mice that were genetically deficient for peptidyl arginine deiminase-4 (PAD4-KO) or DNase1 and 1L3 (DNase1/1L3-DKO). After 48 h, mice were killed. Plasma levels of circulating free DNA (cfDNA) and neutrophil elastase (NE) were quantified to assess NET formation and degradation. Plasma deoxyribonuclease1 (DNase1) protein levels, as well as tissue malondialdehyde (MDA) activity and glutathione peroxidase (GPx) activity, were quantified. DNase1 and DNase1L3 in liver, intestine, spleen, and lung tissues were assessed. The applied sepsis models resulted in a simultaneous increase in NET formation and oxidative stress. NET formation and survival differed in the three models. In contrast to LPS and Volvulus, CLP-induced sepsis showed a decreased and increased 48 h survival in PAD4-KO and DNase1/1L3-DKO mice, when compared to WT mice, respectively. PAD4-KO mice showed decreased formation of NETs and ROS, while DNase1/1L3-DKO mice with impaired NET degradation accumulated ROS and chronicled the septic state. The findings indicate a dual role for NET formation and degradation in sepsis and ischemia-reperfusion (I/R) injury: NETs seem to exhibit a protective capacity in certain sepsis paradigms (CLP model), whereas, collectively, they seem to contribute adversely to scenarios where sepsis is combined with ischemia-reperfusion (volvulus). Full article
(This article belongs to the Special Issue Recent Advances in Neutrophil Extracellular Traps (NETs))
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34 pages, 2218 KiB  
Systematic Review
Farmers’ Transition to Climate-Smart Agriculture: A Systematic Review of the Decision-Making Factors Affecting Adoption
by Marilena Gemtou, Konstantina Kakkavou, Evangelos Anastasiou, Spyros Fountas, Soren Marcus Pedersen, Gohar Isakhanyan, Kassa Tarekegn Erekalo and Serafin Pazos-Vidal
Sustainability 2024, 16(7), 2828; https://doi.org/10.3390/su16072828 (registering DOI) - 28 Mar 2024
Abstract
Agriculture is currently facing major challenges related to ensuring the food security of a rising population and climate change with extreme weather patterns. At the same time, agriculture is a cause of environmental degradation, pollution and biodiversity loss. Climate-smart agriculture (CSA) is proposed [...] Read more.
Agriculture is currently facing major challenges related to ensuring the food security of a rising population and climate change with extreme weather patterns. At the same time, agriculture is a cause of environmental degradation, pollution and biodiversity loss. Climate-smart agriculture (CSA) is proposed as an approach that provides a roadmap to sustainable agricultural development. Despite this, farmer adoption rates of CSA practices and technologies in Europe remain low. This paper seeks to systematically review and synthesize the factors that facilitate or hinder farmers’ uptake of CSA in Europe. Out of the 2827 articles identified in the Web of Science and Scopus databases, a total of 137 research articles were included for analysis following the PRISMA methodology. The factors are categorized into seven categories, namely socio-demographics, psychological, farm characteristics, practice/technology-related attributes, biotic/abiotic, systemic and policy factors, with the majority of the studies focusing on the first four categories, while systemic and policy factors are relatively understudied. The results highlight for the first time that the adoption of CSA does not depend solely on farmer characteristics but also on the food systems and structures in which farmers operate, as well as the interactions with other value chain actors. To promote the adoption of CSA practices, extension and advisory services along with access to timely and reliable information, play a vital role in increasing awareness and in the provision of training and the encouragement of farmers’ behavioral shifts towards sustainable practices. From a technological point of view, adapting technologies to be easy to use, compatible with current farming objectives and cost-efficient will render them less risky investments for farmers and will foster adoption rates. Finally, support from the government in terms of financial support, subsidies and reduced bureaucratic procedures is crucial for motivating CSA adoption. Full article
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9 pages, 535 KiB  
Editorial
Consumer Behavior and Sustainable Marketing Development in Online and Offline Settings
by Sikandar Ali Qalati, Belem Barbosa and Pankaj Deshwal
Sustainability 2024, 16(7), 2829; https://doi.org/10.3390/su16072829 (registering DOI) - 28 Mar 2024
Abstract
The changing dynamics of consumer behavior in today’s digitally driven market presents opportunities and challenges for sustainable marketing development [...] Full article
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25 pages, 3578 KiB  
Article
Operating Properties of Deep Hole Boring Tools with Modified Design
by Norbert K?pczak, Grzegorz Bechciński and Rados?aw Rosik
Materials 2024, 17(7), 1551; https://doi.org/10.3390/ma17071551 (registering DOI) - 28 Mar 2024
Abstract
This paper presents the results of research work on the revised design of a deep hole boring tool. The study was divided into three stages: theoretical, experimental and operational. In the theoretical part, a 3D model of the actual boring bar was created, [...] Read more.
This paper presents the results of research work on the revised design of a deep hole boring tool. The study was divided into three stages: theoretical, experimental and operational. In the theoretical part, a 3D model of the actual boring bar was created, which was subjected to strength tests using the Finite Element Method (FEM), and then prototypes of new deep hole boring tools were made with structural modifications to the shank part of the tool. For the polymer concrete core of a shank, there was a 14.59% lower displacement, and for the rubber-doped polymer concrete (SBR—styrene butadiene rubber) core of a shank there was a 4.84% lower displacement in comparison to the original boring bar. In the experimental part of the study, the original boring bar and the prototypes were subjected to experimental modal analysis and static analysis tests to compare dynamic and static properties. In the operational part of the study, boring tests were carried out for various workpiece materials, during which the basic parameters of the surface geometric structure (SGS), such as roughness Ra and Rz, were studied. Despite the promising preliminary results of the theoretical and experimental studies, using the described modifications to the boring bar is not recommended. Full article
(This article belongs to the Section Manufacturing Processes and Systems)
22 pages, 6787 KiB  
Article
Unlocking Potential: Low Bovine Serum Albumin Enhances the Chondrogenicity of Human Adipose-Derived Stromal Cells in Pellet Cultures
by Isabel Casado-Losada, Melanie Acosta, Barbara Sch?dl, Eleni Priglinger, Susanne Wolbank and Sylvia Nürnberger
Biomolecules 2024, 14(4), 413; https://doi.org/10.3390/biom14040413 (registering DOI) - 28 Mar 2024
Abstract
Bovine serum albumin (BSA) plays a crucial role in cell culture media, influencing cellular processes such as proliferation and differentiation. Although it is commonly included in chondrogenic differentiation media, its specific function remains unclear. This study explores the effect of different BSA concentrations [...] Read more.
Bovine serum albumin (BSA) plays a crucial role in cell culture media, influencing cellular processes such as proliferation and differentiation. Although it is commonly included in chondrogenic differentiation media, its specific function remains unclear. This study explores the effect of different BSA concentrations on the chondrogenic differentiation of human adipose-derived stromal/stem cells (hASCs). hASC pellets from six donors were cultured under chondrogenic conditions with three BSA concentrations. Surprisingly, a lower BSA concentration led to enhanced chondrogenesis. The degree of this effect was donor-dependent, classifying them into two groups: (1) high responders, forming at least 35% larger, differentiated pellets with low BSA in comparison to high BSA; (2) low responders, which benefitted only slightly from low BSA doses with a decrease in pellet size and marginal differentiation, indicative of low intrinsic differentiation potential. In all cases, increased chondrogenesis was accompanied by hypertrophy under low BSA concentrations. To the best of our knowledge, this is the first study showing improved chondrogenicity and the tendency for hypertrophy with low BSA concentration compared to standard levels. Once the tendency for hypertrophy is understood, the determination of BSA concentration might be used to tune hASC chondrogenic or osteogenic differentiation. Full article
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29 pages, 7164 KiB  
Article
Using Integrated Multi-Omics to Explore the Differences in the Three Developmental Stages of Thelephora ganbajun Zang
by Zihan Zhang, Hongzhen Gai and Tao Sha
Appl. Sci. 2024, 14(7), 2856; https://doi.org/10.3390/app14072856 (registering DOI) - 28 Mar 2024
Abstract
Thelephora ganbajun Zang, a rare wild macrofungus, has significant culinary and medicinal value. However, it also has a high cost attributed to its inability to achieve artificial cultivation and its strict environmental requirements. To reveal the intricacies of its development, we conducted a [...] Read more.
Thelephora ganbajun Zang, a rare wild macrofungus, has significant culinary and medicinal value. However, it also has a high cost attributed to its inability to achieve artificial cultivation and its strict environmental requirements. To reveal the intricacies of its development, we conducted a comprehensive analysis of the proteome and metabolome in three pivotal developmental stages: the mycelium, the primordium, and the fruiting body. In our investigation, genes exhibiting various expression levels across multi-omics analyses were identified as potential candidates implicated in growth, development, or metabolic regulation. The aim of this study was to provide a clearer direction for understanding the fundamental metabolic activities and growth stages of this species. Label-free proteomic sequencing revealed a critical juncture in ectomycorrhiza formation, particularly during the transition from the mycelium to the primordium. Secreted proteins, signaling proteins, membrane proteins, and proteins with unidentified functions were rapidly synthesized, with certain amino acids contributing to the synthesis of proteins involved in signaling pathways or hormone precursor substances. In the metabolomics analysis, the classification of secondary metabolites revealed a noteworthy increase in lipid substances and organic acids, contributing to cell activity. The early mycelial development stage exhibited vigorous cell metabolism, contrasting with a decline in cell division activity during fruiting body formation. In our findings, the integration of metabolomic and transcriptomic data highlighted the potential key role of folate biosynthesis in regulating early ectomycorrhiza development. Notably, the expression of alkaline phosphatase and dihydrofolate synthase genes within this pathway was significantly up-regulated in the mycelium and fruiting body stages but down-regulated in the primordium stage. This regulation primarily influences dihydrofolate reductase activity and B vitamin synthesis. Full article
13 pages, 887 KiB  
Article
Blood-Pressure-Lowering and Endothelium-Dependent Vasorelaxant Effects of Nutgall Tree in Rats
by Sujin Shin, Junkyu Park, Ho-Young Choi, Youngmin Bu and Kyungjin Lee
Foods 2024, 13(7), 1041; https://doi.org/10.3390/foods13071041 (registering DOI) - 28 Mar 2024
Abstract
Hypertension is the crucial modifiable risk factor for cardiovascular diseases, and efforts to identify functional foods that are effective for hypertension control are increasing. The nutgall tree (NT, Rhus chinensis Mill.) is used in traditional medicine and food because of its medicinal value. [...] Read more.
Hypertension is the crucial modifiable risk factor for cardiovascular diseases, and efforts to identify functional foods that are effective for hypertension control are increasing. The nutgall tree (NT, Rhus chinensis Mill.) is used in traditional medicine and food because of its medicinal value. However, the role of NT in hypertension has not been investigated. Therefore, the hypotensive effect of NT leaf ethanol extract (NTE) was investigated in spontaneously hypertensive rats (SHRs). SHRs were allocated to three groups (control, 300, or 1000 mg/kg NTE), and blood pressure was measured before and after oral administration. Systolic and diastolic blood pressure significantly decreased in the NTE 1000 mg/kg group and was the lowest at 2 h after administration (−26.4 ± 10.3, −33.5 ± 9.8%, respectively). Daily NTE administration for five days also resulted in a similar effect. Further, the vasorelaxant effects and related mechanisms were investigated in the aortas of Sprague Dawley rats. NTE showed the dose-dependent blood-vessel-relaxing effect, and its mechanism involves the NO-sGC-cGMP pathway, activation of K+ channels, and reduction in the vasoconstrictive action of angiotensin II. Therefore, our study provides basic data indicating the potential use of NTE as a functional food for high blood pressure. Full article
(This article belongs to the Special Issue Plant-Based Foods and Human Health)
9 pages, 772 KiB  
Communication
Impact of Interfering Substances on the Bactericidal Efficacy of Different Commercially Available Hypochlorous Acid-Based Wound Irrigation Solutions Commonly Found in South-East Asia
by Jiann Wen Yap, Neni Iffanida Ismail, Cheng Shoou Lee and Ding Yuan Oh
Antibiotics 2024, 13(4), 309; https://doi.org/10.3390/antibiotics13040309 (registering DOI) - 28 Mar 2024
Abstract
The high prevalence of chronic wounds is a growing concern. Recently, hypochlorous acid (HOCl)-based solutions were introduced as an alternative antimicrobial for wound cleansing. In this study, we assessed the in vitro bactericidal activities of seven commercially available wound irrigation products commonly found [...] Read more.
The high prevalence of chronic wounds is a growing concern. Recently, hypochlorous acid (HOCl)-based solutions were introduced as an alternative antimicrobial for wound cleansing. In this study, we assessed the in vitro bactericidal activities of seven commercially available wound irrigation products commonly found in South-East Asia. The evaluation was conducted using quantitative suspension method, EN 13727 in either low or high protein conditions. Under low protein conditions, four out of the five HOCl products achieved bactericidal activity (≥5 log10 reduction factor; RF) within 2–5 min, and only one product achieved 5 log10 RF at 15 s. None of the HOCl achieved 5 log10 RF under high protein, even after 30 min of exposure time. In contrast, protein interference on the antimicrobial activities of polyhexamethylene biguanide-based product is less pronounced (low protein: 60 s vs. high protein: 2 min to attain ≥5 log10 RF). Octenidine dihydrochloride is the only active not affected by protein interference achieving ≥5 log10 RF within 15 s in both low and high protein conditions. These findings warrant the need to screen antimicrobial wound care products, especially HOCl-based products, in high protein condition to better reflect the antimicrobial activities in wound care. Full article
(This article belongs to the Special Issue Membranes to Fight Drug-Resistant Microbes)
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18 pages, 4951 KiB  
Article
Evaluation of the Potential of Using Machine Learning and the Savitzky–Golay Filter to Estimate the Daily Soil Temperature in Gully Regions of the Chinese Loess Plateau
by Wei Deng, Dengfeng Liu, Fengnian Guo, Lianpeng Zhang, Lan Ma, Qiang Huang, Qiang Li, Guanghui Ming and Xianmeng Meng
Agronomy 2024, 14(4), 703; https://doi.org/10.3390/agronomy14040703 (registering DOI) - 28 Mar 2024
Abstract
Soil temperature directly affects the germination of seeds and the growth of crops. In order to accurately predict soil temperature, this study used RF and MLP to simulate shallow soil temperature, and then the shallow soil temperature with the best simulation effect will [...] Read more.
Soil temperature directly affects the germination of seeds and the growth of crops. In order to accurately predict soil temperature, this study used RF and MLP to simulate shallow soil temperature, and then the shallow soil temperature with the best simulation effect will be used to predict the deep soil temperature. The models were forced by combinations of environmental factors, including daily air temperature (Tair), water vapor pressure (Pw), net radiation (Rn), and soil moisture (VWC), which were observed in the Hejiashan watershed on the Loess Plateau in China. The results showed that the accuracy of the model for predicting deep soil temperature proposed in this paper is higher than that of directly using environmental factors to predict deep soil temperature. In testing data, the range of MAE was 1.158–1.610 °C, the range of RMSE was 1.449–2.088 °C, the range of R2 was 0.665–0.928, and the range of KGE was 0.708–0.885 at different depths. The study not only provides a critical reference for predicting soil temperature but also helps people to better carry out agricultural production activities. Full article
(This article belongs to the Special Issue The Applications of Deep Learning in Smart Agriculture)
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24 pages, 932 KiB  
Article
500 Drivers of S&P 500′s Profitability: Implications for Investment Strategy and Risk Management
by Marek Nagy, Katarina Valaskova, Erika Kovalova and Marcel Macura
Economies 2024, 12(4), 77; https://doi.org/10.3390/economies12040077 (registering DOI) - 28 Mar 2024
Abstract
The financial markets, shaped by dynamic forces, including macroeconomic trends and technological advancements, are influenced by a multitude of factors impacting the S&P 500 stock index, a pivotal indicator in the US equity markets. This paper highlights the significance of understanding the exogenous [...] Read more.
The financial markets, shaped by dynamic forces, including macroeconomic trends and technological advancements, are influenced by a multitude of factors impacting the S&P 500 stock index, a pivotal indicator in the US equity markets. This paper highlights the significance of understanding the exogenous variables affecting the index’s profitability for academics, portfolio managers, and investment professionals. Amid the global ramifications of the S&P 500, particularly in combating the eroding purchasing power caused by inflation, investing in stock indexes emerges as a means to safeguard wealth. The study employs various statistical techniques, emphasizing a methodical approach to uncover influential variables, and using static regression and autoregressive models for immediate and time-lagged effects. In conclusion, the findings have broad practical implications beyond investment strategy, extending to portfolio construction and risk management. Acknowledging inherent uncertainties in financial market forecasts, future research endeavors should target long-term trends, specific influences, and the impact of exchange rate fluctuations on index evolution. Collaboration across regulatory bodies, academia, and the financial industry is underscored, holding the potential for effective risk monitoring and bolstering overall economic and financial market stability. This research serves as a foundational step towards enhancing market understanding and facilitating more efficient investment decision-making approaches. Full article
(This article belongs to the Special Issue Financial Market Volatility under Uncertainty)
12 pages, 29166 KiB  
Article
Surface Modification of Magnetoactive Elastomers by Laser Micromachining
by Izidor Straus, Gaia Kravanja, Luka Hribar, Raphael Kriegl, Matija Jezer?ek, Mikhail Shamonin, Irena Drevensek-Olenik and Ga?per Kokot
Materials 2024, 17(7), 1550; https://doi.org/10.3390/ma17071550 (registering DOI) - 28 Mar 2024
Abstract
It has been recently demonstrated that laser micromachining of magnetoactive elastomers is a very convenient method for fabricating dynamic surface microstructures with magnetically tunable properties, such as wettability and surface reflectivity. In this study, we investigate the impact of the micromachining process on [...] Read more.
It has been recently demonstrated that laser micromachining of magnetoactive elastomers is a very convenient method for fabricating dynamic surface microstructures with magnetically tunable properties, such as wettability and surface reflectivity. In this study, we investigate the impact of the micromachining process on the fabricated material’s structural properties and its chemical composition. By employing scanning electron microscopy, we investigate changes in size distribution and spatial arrangement of carbonyl iron microparticles dispersed in the polydimethylsiloxane (PDMS) matrix as a function of laser irradiation. Based on the images obtained by a low vacuum secondary electron detector, we analyze modifications of the surface topography. The results show that most profound modifications occur during the low-exposure (8 J/cm2) treatment of the surface with the laser beam. Our findings provide important insights for developing theoretical models of functional properties of laser-sculptured microstructures from magnetoactive elastomers. Full article
(This article belongs to the Special Issue Advanced Rubber Composites III)
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20 pages, 823 KiB  
Article
A Numerical Bio-Geotechnical Model of Pressure-Responsive Microbially Induced Calcium Carbonate Precipitation
by Jianye Wang, Helen Mitrani, Anil Wipat, Polly Moreland, Jamie Haystead, Meng Zhang and Martyn Dade Robertson
Appl. Sci. 2024, 14(7), 2854; https://doi.org/10.3390/app14072854 (registering DOI) - 28 Mar 2024
Abstract
The employment of Microbially Induced Calcium Carbonate Precipitation (MICP) is of increasing interest as a technique for environmentally sustainable soil stabilisation. Recent advancements in synthetic biology have allowed for the conception of a pressure-responsive MICP process, wherein bacteria are engineered to sense environmental [...] Read more.
The employment of Microbially Induced Calcium Carbonate Precipitation (MICP) is of increasing interest as a technique for environmentally sustainable soil stabilisation. Recent advancements in synthetic biology have allowed for the conception of a pressure-responsive MICP process, wherein bacteria are engineered to sense environmental loads, thereby offering the potential to stabilise specific soil regions selectively. In this study, a 2D smart bio-geotechnical model is proposed based on a pressure-responsive MICP system. Experimentally obtained pressure-responsive genes and hypothetical genes with different pressure responses were applied in the model and two soil profiles were evaluated. The resulting model bridges scales from gene expression within bacteria cells to geotechnical simulations. The results show that both strata and gene expression–pressure relationships have a significant influence on the distribution pattern of calcium carbonate precipitation within the soil matrix. Among the evaluated experimental genes, Gene A demonstrates the best performance in both of the two soil profiles due to the effective stabilisation in the centre area beneath the load, while Genes B and C are more effective in reinforcing peripheral regions. Furthermore, when the hypothetical genes are utilised, there is an increasing stabilisation area with a decreased threshold value. The results show that the technique can be used for soil reinforcement in specific areas. Full article
(This article belongs to the Special Issue Smart Geotechnical Engineering)
15 pages, 2948 KiB  
Article
SSTR2 Mediates the Inhibitory Effect of SST/CST on Lipolysis in Chicken Adipose Tissue
by Xiao Zhang, Jiannan Zhang, Tianjiao Huang, Xinglong Wang, Jiancheng Su, Jiliang He, Ningkun Shi, Yajun Wang and Juan Li
Animals 2024, 14(7), 1034; https://doi.org/10.3390/ani14071034 (registering DOI) - 28 Mar 2024
Abstract
Somatostatin shows an anti-lipolytic effect in both chickens and ducks. However, its molecular mediator remains to be identified. Here, we report that somatostatin type 2 receptor (SSTR2) is expressed at a high level in chicken adipose tissue. In cultured chicken adipose tissue, the [...] Read more.
Somatostatin shows an anti-lipolytic effect in both chickens and ducks. However, its molecular mediator remains to be identified. Here, we report that somatostatin type 2 receptor (SSTR2) is expressed at a high level in chicken adipose tissue. In cultured chicken adipose tissue, the inhibition of glucagon-stimulated lipolysis by somatostatin was blocked by an SSTR2 antagonist (CYN-154086), supporting an SSTR2-mediated anti-lipolytic effect. Furthermore, a significant pro-proliferative effect was detected in SST28-treated immortalized chicken preadipocytes (ICP-1), and this cell proliferative effect may be mediated through the MAPK/ERK signaling pathway activated by SSTR2. In summary, our results demonstrate that SSTR2 may regulate adipose tissue development by affecting the number and volume of adipocytes in chickens. Full article
(This article belongs to the Collection Poultry Nutrition and Metabolism)
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22 pages, 1774 KiB  
Article
A Rapid Segmentation Method of Highway Surface Point Cloud Data Based on a Supervoxel and Improved Region Growing Algorithm
by Wenshuo Zhao, Yipeng Ning, Xiang Jia, Dashuai Chai, Fei Su and Shengli Wang
Appl. Sci. 2024, 14(7), 2852; https://doi.org/10.3390/app14072852 (registering DOI) - 28 Mar 2024
Abstract
Mobile laser scanning (MLS) systems have become an important technology for collecting and measuring road information for highway maintenance and reconstruction services. However, the efficient and accurate extraction of unstructured road surfaces from MLS point cloud data collected on highways is challenging. Specifically, [...] Read more.
Mobile laser scanning (MLS) systems have become an important technology for collecting and measuring road information for highway maintenance and reconstruction services. However, the efficient and accurate extraction of unstructured road surfaces from MLS point cloud data collected on highways is challenging. Specifically, the complex and unstructured characteristics of road surveying point cloud data lead to traditional 3D point cloud segmentation. When traditional 3D point cloud algorithms extract unstructured road surfaces, over-segmentation and under-segmentation often occur, which affects efficiency and accuracy. To solve these problems, this study introduces an enhanced road extraction method that integrates supervoxel and trajectory information into a traditional region growing algorithm. The method involves two main steps: first, a supervoxel data structure is applied to reconstruct the original MLS point cloud data, which diminishes the calculation time of the point cloud feature vector and accelerates the merging speed of a similar region; second, the trajectory information of the vehicle is used to optimize the seed selection strategy of the regio growing algorithm, which improves the accuracy of road surface extraction. Finally, two typical highway section tests (flat road and slope road) were conducted to validate the positioning performance of the proposed algorithm in an MLS point cloud. The results show that, compared with three kinds of traditional road surface segmentation algorithms, our method achieves an average extraction recall and precision of 99.1% and 96.0%, and by calculating the recall and precision, an F1 score of 97.5% can be obtained to evaluate the performance of the proposed method, for both datasets. Additionally, our method exhibits an average road surface extraction time that is 45.0%, 50.3%, and 55.8% faster than those of the other three automated segmentation algorithms. Full article
9 pages, 1142 KiB  
Article
Epidemiology of Hepatocellular Carcinoma in Taiwan
by Yu-Wei Lai and Ching-Hu Chung
Clin. Pract. 2024, 14(2), 570-578; https://doi.org/10.3390/clinpract14020044 (registering DOI) - 28 Mar 2024
Abstract
Background: Hepatocellular carcinoma (HCC) is a major contributor to the world’s cancer burden. Understanding the HCC incidence rate in Taiwan is thus an interesting avenue of research. Methods: From an NHI database, those patients who had been newly diagnosed with HCC and who [...] Read more.
Background: Hepatocellular carcinoma (HCC) is a major contributor to the world’s cancer burden. Understanding the HCC incidence rate in Taiwan is thus an interesting avenue of research. Methods: From an NHI database, those patients who had been newly diagnosed with HCC and who had been listed on a registry in a catastrophic illness dataset during the years 2013–2021 were enrolled in this study. Antineoplastic agent usage and comorbidities were also studied. Results: The incidence rate of HCC decreased from 57.77 to 44.95 in 100,000 from 2013 to 2021. The average age of patients with HCC increased from 65.54 years old with a CCI score of 4.98 in 2013 to 67.92 years old with a CCI score of 5.49 in 2021. Among these HCC patients, the patients under antineoplastic agent treatment decreased from 53.47% to 31.41% from 2013 to 2021. The presence of comorbidities in HCC patients was about 55.77–83.01% with mild liver disease and 29.93–37.30% with diabetes (without complications) in the period 2013–2021. Conclusions: The incidence rate of HCC slightly decreased in Taiwan. Due to antineoplastic agent usage decreasing over time, these results may indicate that more early-stage HCC patients detected in recent years were mainly treated with surgeries. Full article
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